双调蛋白(AREG)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Amphiregulin (AREG)

AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor

  • 双调蛋白(AREG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 双调蛋白(AREG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对双调蛋白(AREG)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA006Hu01 双调蛋白(AREG)活性蛋白 Cell culture; Activity Assays.
RPA006Hu01 双调蛋白(AREG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA006Hu01 双调蛋白(AREG)多克隆抗体 WB; IHC; ICC; IP.
MAA006Hu22 双调蛋白(AREG)单克隆抗体 WB; IHC; ICC; IP.
MAA006Hu24 双调蛋白(AREG)单克隆抗体 WB; IHC; ICC; IP.
MAA006Hu26 双调蛋白(AREG)单克隆抗体 WB; IHC; ICC; IP.
LAA006Hu76 双调蛋白(AREG)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA006Hu 双调蛋白(AREG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA006Hu 双调蛋白(AREG)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA006Hu01 双调蛋白(AREG)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA006Hu01 双调蛋白(AREG)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Onkologie Amphiregulin and Epiregulin Expression in Colorectal Carcinoma and the Correlation with Clinicopathological Characteristics [Karger: 315380]
Br J Cancer. Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer. [Pubmed:24800946]
Cancer Sci Association between serum ligands and the skin toxicity of anti-epidermal growth factor receptor antibody in metastatic colorectal cancer [PubMed: 25707609]
J Cancer Res Clin Oncol Amphiregulin confers trastuzumab resistance via AKT and ERK activation in HER2-positive breast cancer [PubMed: 26195282]
Oncotarget.  Serum level of hepatocyte growth factor is a novel marker of predicting theoutcome and resistance to the treatment with trastuzumab in HER2-positive patients with metastatic gastric cancer [Pubmed:26716644]
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