血管生成素样蛋白4(ANGPTL4)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Angiopoietin Like Protein 4 (ANGPTL4)

ARP4; FIAF; HFARP; NL2; PGAR; Pp1158; Fasting-Induced Adipose Factor; Hepatic Angiopoietin-Related Protein; Hepatic Fibrinogen/Angiopoietin-Related Protein

  • 血管生成素样蛋白4(ANGPTL4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管生成素样蛋白4(ANGPTL4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对血管生成素样蛋白4(ANGPTL4)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPB019Hu02 血管生成素样蛋白4(ANGPTL4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB019Hu01 血管生成素样蛋白4(ANGPTL4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB019Hu01 血管生成素样蛋白4(ANGPTL4)多克隆抗体 WB; IHC; ICC; IP.
MAB019Hu22 血管生成素样蛋白4(ANGPTL4)单克隆抗体 WB; IHC; ICC; IP.
SEB019Hu 血管生成素样蛋白4(ANGPTL4)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB019Hu 血管生成素样蛋白4(ANGPTL4)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB019Hu01 血管生成素样蛋白4(ANGPTL4)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
The Journal of Biological Chemistry Angiopoietin-like 4 (Angptl4) Protein Is a Physiological Mediator of Intracellular Lipolysis in Murine Adipocytes [PubMed: PMC3318686]
J Clin Endocrinol Metab. Decreased Concentrations of the Lipoprotein Lipase Inhibitor Angiopoietin-Like Protein 4 and Increased Serum Triacylglycerol Are Associated With Increased Neonatal Fat Mass in Pregnant Women With Gestational Diabetes Mellitus [Pubmed: 23744407]
PloS one Lack of Relationship between Cord Serum Angiopoietin-Like Protein 4 (ANGPTL4) and Lipolytic Activity in Human Neonates Born by Spontaneous Delivery [Pubmed: 24324678]
Journal of Hematology & Oncology Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy [PubMed: 26054961]
Gynecol Endocrinol Examination of angiopoietin-like protein 4, neuropeptide Y, omentin-1 levels of obese and non-obese patients with polycystic ovary syndrome [PubMed: 26291814]
Molecular Nutrition & Food research Interaction of Gut Microbiota and High‐sodium, Low‐potassium Diet in Altering Plasma Triglyceride Profiles Revealed by Lipidomics Analysis [Pubmed: 31675161]
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis []
Food Chem Toxicol Di (2-ethylhexyl) phthalate exposure exacerbates metabolic disorders in diet-induced obese mice [34303773]
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