载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Apolipoprotein E (APOE)

Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset

  • 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对载脂蛋白E(APOE)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA704Mu02 载脂蛋白E(APOE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA704Mu01 载脂蛋白E(APOE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA704Mu01 载脂蛋白E(APOE)多克隆抗体 WB; IHC; ICC; IP.
PAA704Mu02 载脂蛋白E(APOE)多克隆抗体 WB; IHC; ICC; IP.
LAA704Mu81 载脂蛋白E(APOE)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA704Mu71 载脂蛋白E(APOE)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA704Mu 载脂蛋白E(APOE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA704Mu 载脂蛋白E(APOE)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA704Mu01 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Seizure - European Journal of Epilepsy Cerebrospinal fluid apolipoprotein E concentration decreases after seizure [Seizure: PIIS1059131109002441]
Biochimica et Biophysica Acta Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ]
Medical Oncology Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma [Pubmed: 23609192]
Diabetes. Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity [Pubmed:25249572]
renal failure Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study [PubMed: 25707516]
Biochim Biophys Acta Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253]
Diabetes Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity [PubMed: 25249572]
Scientific Reports Adaptive immunity against gut microbiota enhances apoE-mediated immune regulation andreduces atherosclerosis and western-diet-related inflammation. [pubmed:27383250]
Biochemical Pharmacology  Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice [Pubmed:30026023]
Oncotarget Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse … [Pubmed:29731975]
Proteomics Clinical Applications Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828]
Nature Communications Hepatic HuR modulates lipid homeostasis in response to high-fat diet [Pubmed: 32546794]
VETERINARY PATHOLOGY Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234]
Journal of Clinical Medicine The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome [33321701]
Cancer Cell Int Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer [33602229]
Nat Commun GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313]
Talanta Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled … [Pubmed:35378356]
Analytica Chimica Acta Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal … [Pubmed:35361429]
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