脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF)

Neurotrophin; Abrineurin

  • 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对脑源性神经营养因子(BDNF)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA011Hu01 脑源性神经营养因子(BDNF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA011Hu61 脑源性神经营养因子(BDNF)活性蛋白 Cell culture; Activity Assays.
EPA011Hu61 脑源性神经营养因子(BDNF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA011Hu01 脑源性神经营养因子(BDNF)多克隆抗体 WB; IHC; ICC; IP.
PAA011Hu06 脑源性神经营养因子(BDNF)多克隆抗体 WB; IHC; ICC; IP.
LAA011Hu71 脑源性神经营养因子(BDNF)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA011Hu81 脑源性神经营养因子(BDNF)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA011Hu22 脑源性神经营养因子(BDNF)单克隆抗体 WB; IHC; ICC; IP.
SEA011Hu 脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA011Hu 脑源性神经营养因子(BDNF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA011Hu01 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Toxicological Sciences Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat Brains [PubMed: 21436126]
Journal of the Neurological Sciences Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord [ScienceDirect: S0022510X12001219]
International Conference on Bioscience Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage [Psrcentre: 212059]
Neuroscience Letters Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice [ScienceDirect: S0304394010015788]
BioMed Research International Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. [NCBI: PMC3925622]
Behavioural Brain Research The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor [Pubmed:25446810]
Neurochemical Research Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. [Pubmed:26559686]
Exp Ther Med. Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats [pmc:PMC4812310]
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
The International Journal af Applied Research in Veterinary Medicine Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study [articles:Vol14Iss3]
J Chem Neuroanat. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. [pubmed:27914953]
Int Immunopharmacol. PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. [pubmed:27940378]
The Journal of Maternal-Fetal & Neonatal Medicine Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639]
Bipolar Disorders Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … [bdi.12449]
Elsevier Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls [science:article]
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