脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF)

Neurotrophin; Abrineurin

  • 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对脑源性神经营养因子(BDNF)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA011Hu04 脑源性神经营养因子(BDNF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA011Hu05 脑源性神经营养因子(BDNF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA011Hu03 脑源性神经营养因子(BDNF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA011Hu01 脑源性神经营养因子(BDNF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA011Hu02 脑源性神经营养因子(BDNF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA011Hu62 脑源性神经营养因子(BDNF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA011Hu01 脑源性神经营养因子(BDNF)活性蛋白 Cell culture; Activity Assays.
APA011Hu61 脑源性神经营养因子(BDNF)活性蛋白 Cell culture; Activity Assays.
EPA011Hu61 脑源性神经营养因子(BDNF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA011Hu01 脑源性神经营养因子(BDNF)多克隆抗体 WB; IHC; ICC; IP.
PAA011Hu02 脑源性神经营养因子(BDNF)多克隆抗体 WB; IHC; ICC; IP.
PAA011Hu06 脑源性神经营养因子(BDNF)多克隆抗体 WB; IHC; ICC; IP.
LAA011Hu71 脑源性神经营养因子(BDNF)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA011Hu81 脑源性神经营养因子(BDNF)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA011Hu22 脑源性神经营养因子(BDNF)单克隆抗体 WB; IHC; ICC; IP.
MAA011Hu21 脑源性神经营养因子(BDNF)单克隆抗体 WB; IHC; ICC; IP.
SEA011Hu 脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA011Hu 脑源性神经营养因子(BDNF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA011Hu 脑源性神经营养因子(BDNF)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA011Hu01 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Toxicological Sciences Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat Brains [PubMed: 21436126]
Journal of the Neurological Sciences Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord [ScienceDirect: S0022510X12001219]
International Conference on Bioscience Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage [Psrcentre: 212059]
Neuroscience Letters Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice [ScienceDirect: S0304394010015788]
BioMed Research International Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. [NCBI: PMC3925622]
Behavioural Brain Research The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor [Pubmed:25446810]
Neurochemical Research Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. [Pubmed:26559686]
Exp Ther Med. Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats [pmc:PMC4812310]
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
The International Journal af Applied Research in Veterinary Medicine Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study [articles:Vol14Iss3]
J Chem Neuroanat. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. [pubmed:27914953]
Int Immunopharmacol. PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. [pubmed:27940378]
The Journal of Maternal-Fetal & Neonatal Medicine Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639]
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease]
Bipolar Disorders Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … [bdi.12449]
Elsevier Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls [science:article]
journal of allergy and clinical immunology Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis [pubmed:28412394]
American Journal of Translational Research A new comorbidity model and the common pathological mechanisms of migraine and epilepsy [pubmed:28559979]
European Journal of Pharmacology Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal. [pubmed:29042206]
Brain, Behavior, and Immunity An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling [Pubmed:29627530]
Stem Cell Research & Therapy Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model [Pubmed:29335016]
Pharmacological Research  N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype [Pubmed:29203442]
Experimental Neurology Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia [Pubmed:30012511]
Nutrients Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model. [Pubmed:29890692]
Neurotoxicity Research Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley Rats [Pubmed:28933048]
Neurotoxicology Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats [Pubmed:29522778]
Neuroscience Letters Long term Westernized diet leads to region-specific changes in brain signaling mechanisms [Pubmed:29655945]
Applied Physiology Nutrition and Metabolism Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclists [Pubmed: 30286300]
Heliyon Establishment and evaluation of a novel mouse model of peri/postmenopausal depression [Doi: 10.1016/j.heliyon.2019.e01195]
BioMed Research International Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling []
International Journal of Immunopathology and Pharmacology Effect of voluntary running on expression of myokines in brains of rats with depression [Pubmed: 30834799]
American Journal of Emergency Medicine Clinical usefulness of brain-derived neurotrophic factor and visinin-like protein-1 in early diagnostic tests for acute stroke [Pubmed: 30826210]
Neurochemistry International Monoaminergic system is implicated in the antidepressant-like effect of hyperoside and protocatechuic acid isolated from Impatiens glandulifera Royle in mice [Pubmed: 31077758]
REVISTA DA ASSOCIACAO MEDICA BRASILEIRA Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with Depression [Pubmed: 30994834]
Journal of Alzheimer's Disease Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer's Disease Model Mice [Pubmed: 31561359]
Biological Trace Element Research Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain Signaling [Pubmed: 31797207]
Psychopharmacology Chronic harmine treatment has a delayed effect on mobility in control and socially defeated rats [Pubmed: 32088835]
Experimental and Therapeutic Medicine Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicity [Pubmed: 32010279]
Journal of Critical Care Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448]
Journal of Clinical Neuroscience CSF levels of a set of neurotrophic factors (brain-derived neurotrophic factor, nerve growth factor) and neuropeptides (neuropeptide Y, galanin) in epileptic children [Pubmed: 32327377]
MOLECULAR NUTRITION & FOOD RESEARCH Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and … [Pubmed: 32918393]
Neuropharmacology Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084]
COLLOIDS AND SURFACES B-BIOINTERFACES Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease [Pubmed: 32777662]
Neurotoxicology Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice [Pubmed: 32522471]
Journal of Veterinary Behavior Comparison of serum brain-derived neurotrophic factor (BDNF) in dogs with and without separation anxiety []
Food & Function Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial … []
Short-but no long-term effect of social stress in rodent depressive-like behavior is not affected by chronic treatment with Harmine []
bioinformatics and biomedical engineering Blood Plasma Trophic Growth Factors Predict the Outcome in Patients with Acute Ischemic Stroke []
Overektomize dişi sıçanlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile nörodavranışsal değişiklikler üzerine anjiyotensin II İnhibisyonunun etkisi []
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­ [33389158]
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways [33589896]
Neuropsychiatr Dis Treat Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567 … [34239303]
Einstein (Sao Paulo) Tropomyosin receptor kinase B-mediated signaling in integration of neuropathic pain and obesity in diabetic polyneuropathy [34586159]
Metab Brain Dis Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men [34762211]
Transl Psychiatry Lithium augmentation of ketamine increases insulin signaling and antidepressant-like active stress coping in a rodent model of treatment-resistant depression [34824208]
Iranian Journal of Basic Medical Sciences Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons [34712429]
J Sex Med Endogenous Deficiency of Brain-Derived Neurotrophic Factor Induces the Downregulation of Tryptophan Hydroxylase-2 Expression in Raphe Nuclei of Rapid … [34384732]
Cell Biochem Funct CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway [34296452]
J Endocrinol Invest Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … [Pubmed:35098494]
Clinical Rheumatology Gender influence on clinical manifestations, depressive symptoms and brain-derived neurotrophic factor (BDNF) serum levels in patients affected by … [Pubmed:35344113]
Sci Rep Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patients [Pubmed:35508633]
Pharmaceutics Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord … [Pubmed:35214053]
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377]
Journal of Complementary Medicine Research Effect of herbal formulation Sharbat Ahmad Shahi on serum BDNF level in mild to moderate cases of depressive disorder []
AFRICAN JOURNAL OF BIOCHEMISTRY RESEARCH Medicinal effects of Saffron on obese animals by reducing Castellis risk index to protect from cardio-pathology and psychological distress []
留言咨询