纤连蛋白(FN)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Fibronectin (FN)

FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein

  • 纤连蛋白(FN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 纤连蛋白(FN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对纤连蛋白(FN)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
NPA037Bo01 纤连蛋白(FN)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA037Bo01 纤连蛋白(FN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA037Bo01 纤连蛋白(FN)多克隆抗体 WB; IHC; ICC; IP.
LAA037Bo71 纤连蛋白(FN)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA037Bo21 纤连蛋白(FN)单克隆抗体 WB; IHC; ICC; IP.
SEA037Bo 纤连蛋白(FN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA037Bo 纤连蛋白(FN)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA037Bo01 纤连蛋白(FN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Journal of Ethnopharmacology Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy [ScienceDirect: S0378874114002414]
Investigative Ophthalmology & Visual Science Inhibition by Female Sex Hormones of Collagen Gel Contraction Mediated by Retinal Pigment Epithelial Cells [Pubmed: 24609629]
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592]
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747]
Laryngoscope. Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625]
Biomedical Materials In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132]
Biomedical Materials In vivo [PubMed: 25634132]
American Journal of Medical and Biological Research Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate onNF-κB/leptin-insulin Axisin Obese Subjects with Non-alcoholic Fatty Liver Disease [Ajmbr: 3]
Food Chem. Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528]
journal of perinatal medicine Maternal endothelial damage as a disorder shared by early preeclampsia, late preeclampsia and intrauterine growth restriction. [pubmed:27865093]
Chinese Journal of Contemporary Neurology and Neurosurgery Effect of panax notoginseng saponins on efficacy and hemorrhagic transformation of rt-PA intravenous thrombolysis in patients with acute ischemic stroke [Article_en:CJFDTotal-XDJB201611014]
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