血红蛋白(HB)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Hemoglobin (HB)
Hgb; Haemoglobin; Heterotetramer(αβ)2
- 编号KSB409Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma and erythrocyte lysates
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.55-400ng/mL
- 可适灵敏度0.24ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 4298 ¥ 7164 ¥ 12895 ¥ 25074 ¥ 42984
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特异性
试剂盒中的抗体对血红蛋白(HB)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
NPB409Mu01 | 血红蛋白(HB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB409Mu01 | 血红蛋白(HB)多克隆抗体 | WB; IHC; ICC; IP. |
LAB409Mu91 | 血红蛋白(HB)多克隆抗体(辣根过氧化物酶标记) | WB; IHC; ICC. |
SEB409Mu | 血红蛋白(HB)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
KSB409Mu01 | 血红蛋白(HB)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna [Pubmed:25460046] |
PLoS One. | Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis [Pubmed:27027307] |
Parasites & Vectors | The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis. [pubmed:27561965] |
Proteomics. Clinical Applications | Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women. [pubmed:27615121] |
Molecular nutrition & food research | Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro [pubmed:28105758] |
Frontiers in Immunology | Oxidized Hemoglobin Is Antigenic and Immunogenic in Lupus [pubmed:28694810] |
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