血幼素(HJV)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Hemojuvelin (HJV)

HFE2; HFE2A; HJV; JH; RGMC; hemochromatosis type 2(Juvenile); Haemojuvelin; RGM domain family member C

  • 血幼素(HJV)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 血幼素(HJV)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对血幼素 (HJV)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB995Ra01 血幼素(HJV)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB995Ra01 血幼素(HJV)多克隆抗体 WB; IHC; ICC; IP.
MAB995Ra21 血幼素(HJV)单克隆抗体 WB; IHC; ICC; IP.
SEB995Ra 血幼素(HJV)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB995Ra 血幼素(HJV)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB995Ra01 血幼素(HJV)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
PLoS One. Effect of Iron Overload and Iron Deficiency on Liver Hemojuvelin Protein [PubMed: PMC3356351]
Journal of Hepcidin Levels Are Increased in Patients with Acute Ischemic Stroke: Preliminary Report [PubMed: 25881778]
European Journal of Haematology Soluble hemojuvelin in transfused and untransfused thalassaemic subjects [doi:10.1111]
American Journal of Nephrology The secret war against counterfeit science [pubmed:28492262]
Critical Care Urinary biomarkers predict advanced acute kidney injury after cardiovascular surgery [Pubmed:29699579]
Scientific Reports Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery [Pubmed:29386545]
Journal of Cerebral Blood Flow and Metabolism The functional role of hemojuvelin in acute ischemic stroke [Pubmed: 31307288]
Oxidative damage and iron dyshomeostasis in thalassemic patients []
J Trace Elem Med Biol Caffeine and alcohol-friends or foes of human iron stores? [Pubmed:35063815]
Arch Pathol Lab Med Urinary Biomarkers Can Predict Weaning From Acute Dialysis Therapy in Critically Ill Patients [Pubmed:35311928]
Arch Pathol Lab Med The Relationships among Plasma Fetuin-B, Thyroid Autoimmunity, and Fertilization Rate In Vitro Fertilization and Embryo Transfer [Pubmed:35340679]
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