异柠檬酸脱氢酶1(IDH1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Isocitrate Dehydrogenase 1, Soluble (IDH1)

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase

  • 异柠檬酸脱氢酶1(IDH1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 异柠檬酸脱氢酶1(IDH1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对异柠檬酸脱氢酶1(IDH1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Molecular & Cellular Proteomics Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic Analysis [Mcponline: 008821]
Molecular & Cellular Proteomics Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic Analysis [Pubmed: 22064513]
Clinical Cancer Research Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancer [Pubmed: 24046070]
Onco Targets Ther The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cells [PubMed: 25914546]
Oncogene Metformin sensitizes endometrial cancer cells to chemotherapy through IDH1-induced Nrf2 expression via an epigenetic mechanism [Pubmed:29921847]
Molecular Oncology IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancer [Pubmed:29465809]
Cancers Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver … [Pubmed: 32443439]
International Journal of Cancer Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer [Pubmed:35689436]
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