乳铁传递蛋白(LTF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Lactoferrin (LTF)

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

  • 乳铁传递蛋白(LTF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 乳铁传递蛋白(LTF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对乳铁传递蛋白(LTF)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA780Hu01 乳铁传递蛋白(LTF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA780Hu02 乳铁传递蛋白(LTF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA780Hu01 乳铁传递蛋白(LTF)多克隆抗体 WB; IHC; ICC; IP.
PAA780Hu02 乳铁传递蛋白(LTF)多克隆抗体 WB; IHC; ICC; IP.
LAA780Hu81 乳铁传递蛋白(LTF)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA780Hu71 乳铁传递蛋白(LTF)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA780Hu22 乳铁传递蛋白(LTF)单克隆抗体 WB; IHC; ICC; IP.
LAA780Hu72 乳铁传递蛋白(LTF)单克隆抗体(生物素标记) WB; IHC; ICC.
LAA780Hu82 乳铁传递蛋白(LTF)单克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEA780Hu 乳铁传递蛋白(LTF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA780Hu 乳铁传递蛋白(LTF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA780Hu01 乳铁传递蛋白(LTF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
The International Journal of Biochemistry & Cell Biology Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reaction [ScienceDirect: S1357272511002524]
BMC Veterinary Research Changes in composition of colostrum of Egyptian buffaloes and Holstein cows [BioMed: 17466148]
Plos One p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation [PlosOne: source]
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy [Pubmed:25505239]
PLoS One Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4 [Pubmed:26974438]
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