粘蛋白2(MUC2)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Mucin 2 (MUC2)
MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal
- 编号KSA705Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本tissue homogenates, cell lysates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.113ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对粘蛋白2(MUC2)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA705Hu01 | 粘蛋白2(MUC2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA705Hu01 | 粘蛋白2(MUC2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA705Hu71 | 粘蛋白2(MUC2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA705Hu21 | 粘蛋白2(MUC2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA705Hu24 | 粘蛋白2(MUC2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA705Hu22 | 粘蛋白2(MUC2)单克隆抗体 | WB |
| SEA705Hu | 粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA705Hu | 粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA705Hu01 | 粘蛋白2(MUC2)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Annals of surgical oncology | Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. [PubMed: 20717734] |
| World J Gastroenterol. | Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats. [Pubmed:24627600] |
| Front Microbiol | Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323] |
| nutrition research and practice | Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway [PubMed: 26244071] |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851] |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513] |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489] |
| Bio-protocol | Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA [10.21769:BioProtoc.2879] |
| Journal of Investigative Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494] |
| Biomolecules | Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast Cancer [Pubmed: 30682816] |
| Cell Stem Cell | PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism [Pubmed: 31564549] |
| DIGESTIVE DISEASES AND SCIENCES | Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats [Pubmed: 32144600] |
| Scientific Reports | Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli [Pubmed: 32601325] |
| Biorxiv | Defective intestinal repair after short-term high fat diet due to loss of efferocytosis [] |
| J Cell Mol Med | Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis [34145945] |
| Cell Tissue Res | Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced … [34370080] |
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