肾足蛋白(NPHN)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Nephrin (NPHN)

NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor

  • 肾足蛋白(NPHN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 肾足蛋白(NPHN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对肾足蛋白(NPHN)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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KSA937Ra01 肾足蛋白(NPHN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Yonsei medical journal The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia. [pubmed:28120572]
Experimental and molecular pathology Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. [pubmed:29246788]
British Journal of Haematology  Renal protection by atorvastatin in a murine model of sickle cell nephropathy [Pubmed:29527679]
Journal of Clinical Medicine Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease [Pubmed: 32961903]
Disease Markers Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study [34707724]
Biomed Rep Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs [Pubmed:35620307]
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