95 KSA899Hu01 | 骨桥素(OPN)检测试剂盒DIY材料(酶联免疫吸附试验法) | Homo sapiens (Human,人) USCN(武汉优尔生商贸有限公司)

骨桥素(OPN)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Osteopontin (OPN)

SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 骨桥素(OPN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 骨桥素(OPN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对骨桥素(OPN)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用
EPA899Hu61 骨桥素(OPN)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA899Hu02 骨桥素(OPN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA899Hu61 骨桥素(OPN)活性蛋白 Cell culture; Activity Assays.
PAA899Hu06 骨桥素(OPN)多克隆抗体 WB; IHC; ICC; IP.
PAA899Hu02 骨桥素(OPN)多克隆抗体 WB; IHC; ICC; IP.
LAA899Hu71 骨桥素(OPN)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA899Hu22 骨桥素(OPN)单克隆抗体 WB; IHC; ICC; IP.
SEA899Hu 骨桥素(OPN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA899Hu 骨桥素(OPN)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA899Hu01 骨桥素(OPN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Journal of Biomedical Materials Research Part A Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture [PubMed: 19280635]
Osteoarthritis and Cartilage Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients [PubMed: 19747583]
The Journal of Rheumatology Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. [Jrheum: source]
International Journal of Pharmaceutics Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester) [ScienceDirect: S0378517312003742]
European Cells and matreials The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering [Ecmjournal: Source]
Journal of Equine Veterinary Science Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse [ScienceDirect: Source]
Basic & Clinical Pharmacology & Toxicology Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions [Wiley:Source]
Journal of Cellular Biochemistry Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts [Pubmed:24905552]
Naunyn-Schmiedeberg's Archives of Pharmacology Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats [Pubmed:25038619]
J Endocrinol Invest.? The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337]
FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells [Pubmed:25542352]
Gene Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis [PubMed: 26283150]
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
Journal of Biomaterials and Tissue Engineering Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel [Content: Asp]
Primasari Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer [Index.Php: Amor]
Int J Biol Sci Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway [PubMed: 26327817]
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma. [Ebscohost]
Am J Pathol.  Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. [Pubmed:26963343]
circulation journal Stenotic Bicuspid and Tricuspid Aortic Valves - Micro-Computed Tomography and Biological Indices of Calcification. [pubmed:28344201]
Journal of Cardiovascular Electrophysiology Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. [pubmed:28127817]
American Journal of Translational Research Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes. [pubmed:28337262]
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