降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Procalcitonin (PCT)

Pro-Calcitonin

  • 降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对降钙素原(PCT)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Scandinavian Journal of Infectious Diseases Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis [PubMed: 20085423]
International Journal of Radiation Oncology ? Biology ? Physics Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice [PubMed: 20637981]
Ulus Travma Acil Cerrahi Derg Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia [PubMed: 21935794]
Egyptian Journal of Bronchology Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children [Essbronchology: Source]
University of Waikato The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis [Waikato:Source]
Egyptian Journal of Medical Microbiology Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis [Ejmm:Source]
Nigerian Medical Journal Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis [Pubmed:25657488]
Wiley online library The use and value of procalcitonin in solid organ transplantation [PubMed: 25996831]
Balkan Med J Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia [PubMed: 26185718]
The Journal of Clinical Endocrinology & Metabolism Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay [PubMed: 26647152]
Journal of Human Hypertension Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension [PubMed: 26040439]
Medical Science Monitor Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection. [Pubmed:26772168]
International Immunopharmacology Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602]
Pancreatology. Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. [Pubmed:26804005]
Acta Medica Risk Factors of Acute Pancreatitis in Oral Double Balloon Enteroscopy. [pubmed:27638962]
The Clinical Respiratory Journal Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome [pubmed:28960823]
SHOCK INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION [pubmed:27984536]
Cellular Physiology and Biochemistry Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure [Pubmed:29913443]
Archives of Medical Science Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase [Pubmed:29593801]
Journal of Thoracic Disease Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung [Pubmed:29707313]
Asian Journal of Psychiatry Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients [Pubmed:29723719]
European Review for Medical and Pharmacological Sciences The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341]
Medicina Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection [Pubmed: 33080909]
MEDIATORS OF INFLAMMATION Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase [Pubmed: 32410851]
Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes []
Journal of Immunology Research Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis [33628853]
Journal of Medical Biochemistry Circulating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer [33776564]
Surg Endosc Original contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesity [Pubmed:35044516]
Bone Res Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment [Pubmed:35087025]
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