抵抗素(RETN)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Resistin (RETN)

ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3

  • 抵抗素(RETN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 抵抗素(RETN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对抵抗素(RETN)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA847Mu01 抵抗素(RETN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA847Mu02 抵抗素(RETN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA847Mu01 抵抗素(RETN)多克隆抗体 WB; IHC; ICC; IP.
PAA847Mu02 抵抗素(RETN)多克隆抗体 WB; IHC; ICC; IP.
LAA847Mu81 抵抗素(RETN)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA847Mu71 抵抗素(RETN)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA847Mu21 抵抗素(RETN)单克隆抗体 WB; IHC; ICC; IP.
SEA847Mu 抵抗素(RETN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA847Mu 抵抗素(RETN)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA847Mu01 抵抗素(RETN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
European Journal of Pharmacology Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet [PubMed: 20869961]
Evid Based Complement Alternat Med. Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats [Pubmed:Pmc4363645]
J Endocrinol. Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle [Pubmed:24681828]
Life Sciences Adipose tissue regulates hepatic cholesterol metabolism via adiponectin [Pubmed:25445438]
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126]
Evid Based Complement Alternat Med Tinospora crispa [PubMed: 25821506]
Journal of Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysis [PubMed: 26686707]
Genome Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without Complications [PubMed: 25120108]
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines [science:S0065128116301234]
Journal of Dairy Science Endocrine activity of adipose tissues as influenced by energy intake in the periparturient cow [108495429X:34]
Evidence-Based Complementary and Alternative Medicine Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. [pubmed:28246535]
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