转化生长因子α(TGFa)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Transforming Growth Factor Alpha (TGFa)

ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF

  • 转化生长因子α(TGFa)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 转化生长因子α(TGFa)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对转化生长因子α(TGFa)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Journal of Physiology and Biochemistry Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats [SpringerLink: h875w73171w2147w]
Oncology Reports A bone-seeking clone exhibits different biological properties from the ACHN parental human renal cell carcinoma in vivo and in vitro [PubMed: PMC3583590]
Sci Total Environ. The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female. [pubmed:28174048]
International Journal of Biological Sciences CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17 []
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets [Pubmed: 33164313]
Toll-like receptor 5-mediated signaling enhances liver regeneration in mice []
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