血管内皮生长因子C(VEGFC)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Vascular Endothelial Growth Factor C (VEGFC)

VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein

  • 血管内皮生长因子C(VEGFC)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管内皮生长因子C(VEGFC)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对血管内皮生长因子C(VEGFC)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA145Ra01 血管内皮生长因子C(VEGFC)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA145Ra01 血管内皮生长因子C(VEGFC)多克隆抗体 WB; IHC; ICC; IP.
LAA145Ra81 血管内皮生长因子C(VEGFC)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA145Ra71 血管内皮生长因子C(VEGFC)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA145Ra22 血管内皮生长因子C(VEGFC)单克隆抗体 WB
MAA145Ra21 血管内皮生长因子C(VEGFC)单克隆抗体 WB; IHC; ICC; IP.
LAA145Ra72 血管内皮生长因子C(VEGFC)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA145Ra 血管内皮生长因子C(VEGFC)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA145Ra 血管内皮生长因子C(VEGFC)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSA145Ra01 血管内皮生长因子C(VEGFC)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA145Ra01 血管内皮生长因子C(VEGFC)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Journal of Cellular Biochemistry Alternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinoma [PubMed: 19241443]
genetics and molecular research Expression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stages [PubMed: 26125824]
Experimental and Therapeutic Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxia [PubMed: 26622498]
Am J Pathol IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via Macrophages [PubMed: 26608451]
PLoS One. Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells. [pubmed:27383632]
Oncology Letters RNAi-mediated gene silencing of vascular endothelial growth factor C suppresses growth andinduces apoptosis in mouse breast cancer in vitro and in vivo. [pubmed:27895746]
Angiogenesis Characterization of isolated liver sinusoidal endothelial cells for liver bioengineering [Pubmed:29582235]
Oncology Reports Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells [Pubmed:29328465]
LABORATORY INVESTIGATION Dynamic signature of lymphangiogenesis during acute kidney injury and chronic kidney disease [Pubmed: 31019289]
Cutaneous and Ocular Toxicology Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions? [Pubmed: 32722957]
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