维生素D结合蛋白(DBP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Vitamin D Binding Protein (DBP)

GC; VDBG; VDBP; Group-Specific Component; Gc-globulin

  • 维生素D结合蛋白(DBP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 维生素D结合蛋白(DBP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对维生素D结合蛋白(DBP)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB810Ra02 维生素D结合蛋白(DBP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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PAB810Ra02 维生素D结合蛋白(DBP)多克隆抗体 WB; IHC; ICC; IP.
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LAB810Ra71 维生素D结合蛋白(DBP)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB810Ra22 维生素D结合蛋白(DBP)单克隆抗体 WB; IHC; ICC; IP.
SEB810Ra 维生素D结合蛋白(DBP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB810Ra01 维生素D结合蛋白(DBP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Proteome Science Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patients [PubMed: 23101585]
Toxins (Basel). Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278]
Endocrine Vitamin D-binding protein and free vitamin D concentrations in acromegaly [PubMed: 26547217]
Proteomics Clinical Applications Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509]
PLoS One. 25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is Associated with Disease Activity in Systemic Lupus Erythematosus Patients. [pubmed:28085957]
PROTEOMICS - Clinical Applications Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. [pubmed:26680509]
Plos One 25-Hydroxivitamin D serum concentration, not free and bioavailable vitamin D, is associated with disease activity in systemic lupus erythematosus patients [10.1371/journal.pone.0170323]
Nutrients Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … [Pubmed:30037068]
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