Active 5'-Nucleotidase, Ecto (NT5E)
CD73; NT5-E; E5NT; E5-NT; NTE; EN; ENT
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 90%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1728 ¥ 4320 ¥ 8640 ¥ 25920 ¥ 64800
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant NT5E, Human.
- 通过ISO 9001、ISO 13485质量体系认证
5'-Nucleotidase, Ecto (NT5E), also known as ecto-5'-nucleotidase or CD73, is an enzyme catalyzing thehydrolysis of nucleoside-5’-monophosphates to nucleosides and inorganic phosphate. The enzyme is a dimer composed of 2 identical 70kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. NT5E is a marker of lymphocyte differentiation that has functions independent of its catalytic activity, such as T-cell activation and cell-cell adhesion. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from NT5E by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The enzyme is widely distributed in human and animal tissues. Besides, AF4/FMR2 Family, Member 1 (AFF1) has been identified as an interactor of NT5E thus a binding ELISA assay was conducted to detect the interaction of recombinant human NT5E and recombinant human AFF1. Briefly, NT5E were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to AFF1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NT5E pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of NT5E and AFF1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|APB250Hu01||胞外5'-核苷酸酶(NT5E)活性蛋白||Cell culture; Activity Assays.|
|RPB250Hu01||胞外5'-核苷酸酶(NT5E)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB250Hu01||胞外5'-核苷酸酶(NT5E)多克隆抗体||WB; IHC; ICC; IP.|
|LAB250Hu81||胞外5'-核苷酸酶(NT5E)多克隆抗体(异硫氰酸荧光素标记)||WB; IHC; ICC; IF.|
|LAB250Hu71||胞外5'-核苷酸酶(NT5E)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAB250Hu21||胞外5'-核苷酸酶(NT5E)单克隆抗体||WB; IHC; ICC; IP.|
|MAB250Hu22||胞外5'-核苷酸酶(NT5E)单克隆抗体||WB; IHC; ICC; IP.|
|SEB250Hu||胞外5'-核苷酸酶(NT5E)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|Journal of Autoimmunity||The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation [Pubmed:25640206]|