胞外5'-核苷酸酶(NT5E)活性蛋白

Active 5'-Nucleotidase, Ecto (NT5E)

CD73; NT5-E; E5NT; E5-NT; NTE; EN; ENT

  • 胞外5'-核苷酸酶(NT5E)活性蛋白 产品包装(模拟)
  • 胞外5'-核苷酸酶(NT5E)活性蛋白 产品包装(模拟)
  • 胞外5'-核苷酸酶(NT5E)活性蛋白 Gene sequencing
  • APB250Hu01.jpg SDS-PAGE图
  • 胞外5'-核苷酸酶(NT5E)活性蛋白 Figure. Western Blot; Sample: Recombinant NT5E, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

5'-Nucleotidase, Ecto (NT5E), also known as ecto-5'-nucleotidase or CD73, is an enzyme catalyzing thehydrolysis of nucleoside-5’-monophosphates to nucleosides and inorganic phosphate. The enzyme is a dimer composed of 2 identical 70kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. NT5E is a marker of lymphocyte differentiation that has functions independent of its catalytic activity, such as T-cell activation and cell-cell adhesion. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from NT5E by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The enzyme is widely distributed in human and animal tissues. Besides, AF4/FMR2 Family, Member 1 (AFF1) has been identified as an interactor of NT5E thus a binding ELISA assay was conducted to detect the interaction of recombinant human NT5E and recombinant human AFF1. Briefly, NT5E were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to AFF1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NT5E pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of NT5E and AFF1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB250Hu01 胞外5'-核苷酸酶(NT5E)活性蛋白 Cell culture; Activity Assays.
RPB250Hu01 胞外5'-核苷酸酶(NT5E)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB250Hu01 胞外5'-核苷酸酶(NT5E)多克隆抗体 WB; IHC; ICC; IP.
FAB250Hu01 抗胞外5'-核苷酸酶(NT5E)多克隆抗体 Flow cytometry.
LAB250Hu81 胞外5'-核苷酸酶(NT5E)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB250Hu71 胞外5'-核苷酸酶(NT5E)多克隆抗体(生物素标记) WB; IHC; ICC.
FAB250Hu41 抗胞外5'-核苷酸酶(NT5E)多克隆抗体(藻红素标记) Flow cytometry.
FAB250Hu51 抗胞外5'-核苷酸酶(NT5E)多克隆抗体(别藻蓝蛋白标记) Flow cytometry.
FAB250Hu81 抗胞外5'-核苷酸酶(NT5E)多克隆抗体(异硫氰酸荧光素标记) Flow cytometry.
MAB250Hu21 胞外5'-核苷酸酶(NT5E)单克隆抗体 WB; IHC; ICC; IP.
MAB250Hu22 胞外5'-核苷酸酶(NT5E)单克隆抗体 WB; IHC; ICC; IP.
SEB250Hu 胞外5'-核苷酸酶(NT5E)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB250Hu01 胞外5'-核苷酸酶(NT5E)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Journal of Autoimmunity The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation [Pubmed:25640206]
留言咨询