酸性神经鞘磷脂酶(ASM)活性蛋白

Active Acid Sphingomyelinase (ASM)

SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase

  • 酸性神经鞘磷脂酶(ASM)活性蛋白 产品包装(模拟)
  • 酸性神经鞘磷脂酶(ASM)活性蛋白 产品包装(模拟)
  • 酸性神经鞘磷脂酶(ASM)活性蛋白 Figure . Gene Sequencing (extract)
  • APB360Hu61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), belongs to the sphingomyelin phosphodiesterase family. The protein Converts sphingomyelin to ceramide. ASM also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.
Thus, the recombinant human ASM activity was measured by its ability to hydrolyze 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HDA-PC) to p-Nitrophenol. The reaction was performed in 50 mM MES, 0.5 µM ZnCl2, pH 7.5 ( Assay Buffer), initiated by addition 50 μL of various concentrations of ASM (diluted with Assay Buffer) to 50 µL of 1 mM Substrate HDA-PC ( 50 mM stock solution in methanol, diluted with Assay Buffer). Incubated at room temperature for 20 minutes in the dark, then read at a wavelength of 405 nm.

One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of HDA-PC to p-Nitrophenol in 1min at 37°C. The specific activity of recombinant human ASM is >1000 pmol/min/µg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
EPB360Hu61 酸性神经鞘磷脂酶(ASM)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB360Hu01 酸性神经鞘磷脂酶(ASM)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB360Hu61 酸性神经鞘磷脂酶(ASM)活性蛋白 Cell culture; Activity Assays.
PAB360Hu01 酸性神经鞘磷脂酶(ASM)多克隆抗体 WB; IHC; ICC; IP.
MAB360Hu22 酸性神经鞘磷脂酶(ASM)单克隆抗体 WB; IHC; ICC; IP.
SEB360Hu 酸性神经鞘磷脂酶(ASM)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB360Hu 酸性神经鞘磷脂酶(ASM)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB360Hu 酸性神经鞘磷脂酶(ASM)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Blood.? Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD [Pubmed:25075126]
Journal of Clinical Lipidology Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes [10.1016/j.jacl.2017.03.007]
Diabetes & metabolism Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? [pubmed:28499696]
INFLAMMATION Acid Sphingomyelinase and Acid ¦Â-Glucosidase 1 Exert Opposite Effects on Interleukin-1¦Â-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes [33665756]
Indian J Ophthalmol Evaluation of sphingolipid metabolism on diabetic retinopathy [34708809]
留言咨询