激活素A(ACVA)活性蛋白

Active Activin A (ACVA)

ACV-A; Activin Beta A Beta A Homodimer

  • 激活素A(ACVA)活性蛋白 产品包装(模拟)
  • 激活素A(ACVA)活性蛋白 产品包装(模拟)
  • APA001Hu01.jpg Figure. SDS-PAGE
  • 激活素A(ACVA)活性蛋白 WB图
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活性实验

Activin A (ACVA) is a member of the TGF-β superfamily of cytokines and involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis. To test the effect of ACVA on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 3,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant human ACVA. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2 hours at 37℃. Proliferation of A549 cells after incubation with ACVA for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human ACVA for 48h. The result was shown in Figure 2. It was obvious that ACVA significantly inhibit cell viability of A549 cells. The ED50 is 5.09 μg/mL.

Figure 2. Inhibition of A549 cells proliferation after stimulated with ACVA.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA001Hu01 激活素A(ACVA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA001Hu01 激活素A(ACVA)活性蛋白 Cell culture; Activity Assays.
PAA001Hu01 激活素A(ACVA)多克隆抗体 WB; ICC; IHC-P; IHC-F; ELISA.
SEA001Hu 激活素A(ACVA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA001Hu 激活素A(ACVA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA001Hu 激活素A(ACVA)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA001Hu 激活素A(ACVA)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
13 Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction [Ahajournals: Source]
J. Pharmacogn [Article: Hksobf_2015_V46N2_167]
United States Patent Application Methods for Diagnosis and Treatment of Concussion or Brain Injury [2016:0103140]
BMC Cancer. Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. [pubmed:27835986]
Basic Clin Pharmacol Toxicol. Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. [pubmed:28032440]
Basic & Clinical Pharmacology & Toxicology Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling [doi:10.1111]
Journal of Immunology MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis Infection [Pubmed:29661829]
Journal of Vascular Surgery Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissection [Pubmed:29685513]
Acta Biochimica et Biophysica Sinica Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis [10.1093:abbs]
Poultry Science The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeys [Pubmed:29917099]
Food Bioscience Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity [10.1016:j.fbio.2018.06.002]
Current Analytical Chemistry Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A [10.2174:2211556006666170525161225]
Journal of Veterinary Internal Medicine FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB [:]
Universal Journal of Pharmaceutical Research SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN [10.22270:ujpr.v3i2.138]
Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention [:]
Clin Oral Investig.  Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitis [Pubmed:29713888]
Myocardial fibrosis in hypertrophic cardiomyopathy [:]
TELLING TRANSVERSAL COMPETENCES… TO BE PROFESSIONALLY PROMOTED [:]
Protein biomarker panels for detecting colorectal cancer and advanced adenoma [:]
Endothelotropic activity of 4-hydroxy-3, 5-di-tret-butylcinnamic acid in the conditions of experimental cerebral ischemia [:]
Silencing of the CSNK2β gene by siRNA inhibits invasiveness and growth of MDA-MB-231 cells [10.1101:311092]
Duchenne Muscular Dystrophy Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy [Pubmed:29067667]
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Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517]
clinica chimica acta The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control study [Pubmed: 31647905]
hindawi The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients []
Pathology & Oncology Research Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status [34867090]
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