Active Adenosylhomocysteinase (AHCY)
SAHH; AdoHcyase; S-Adenosylhomocysteine Hydrolase; S-adenosyl-L-homocysteine hydrolase
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1944 ¥ 4860 ¥ 9720 ¥ 29160 ¥ 72900
- Figure. SDS-PAGE
- Figure. Western Blot
- 通过ISO 9001、ISO 13485质量体系认证
Figure. The binding activity of AHCY with MHCB.
Adenosylhomocysteinase (S-adenosylhomocysteine synthase, S-adenosylhomocysteine hydrolase, adenosylhomocysteine hydrolase, S-adenosylhomocysteinase, SAHase, AdoHcyase, AHCY) is an enzyme that converts S-adenosylhomocysteine to homocysteine and adenosine. The enzyme contains one tightly bound NAD per subunit. Besides, Major Histocompatibility Complex Class I B (MHCB) has been identified as an interactor of AHCY, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AHCY and recombinant human MHCB. Briefly, AHCY were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCB-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AHCY pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of AHCY and MHCB was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|RPG465Hu01||腺苷高半胱氨酸水解酶(AHCY)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APG465Hu01||腺苷高半胱氨酸水解酶(AHCY)活性蛋白||Cell culture; Activity Assays.|
|PAG465Hu01||腺苷高半胱氨酸水解酶(AHCY)多克隆抗体||WB; IHC; ICC; IP.|
|MAG465Hu21||腺苷高半胱氨酸水解酶(AHCY)单克隆抗体||WB; IHC; ICC; IP.|
|SEG465Hu||腺苷高半胱氨酸水解酶(AHCY)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|