载脂蛋白A1(APOA1)活性蛋白

Active Apolipoprotein A1 (APOA1)

Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I

  • 载脂蛋白A1(APOA1)活性蛋白 产品包装(模拟)
  • 载脂蛋白A1(APOA1)活性蛋白 产品包装(模拟)
  • 载脂蛋白A1(APOA1)活性蛋白 Figure. Gene Sequencing (Extract)
  • APA519Hu01.png Figure. SDS-PAGE
  • 载脂蛋白A1(APOA1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of APOA1 with Hpt.
Apolipoprotein A1 (APOA1) is the major protein component of HDL particles in plasma. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA1 was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. ApoA1 is often used as a biomarker for prediction of cardiovascular diseases. Besides, Haptoglobin (Hpt) has been identified as an interactor of APOA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APOA1 and recombinant human Hpt. Briefly, APOA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Hpt-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-APOA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of APOA1 and Hpt was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA519Hu01 载脂蛋白A1(APOA1)活性蛋白 Cell culture; Activity Assays.
RPA519Hu01 载脂蛋白A1(APOA1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA519Hu61 载脂蛋白A1(APOA1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA519Hu01 载脂蛋白A1(APOA1)多克隆抗体 WB; IHC; ICC; IP.
LAA519Hu71 载脂蛋白A1(APOA1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA519Hu21 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC
MAA519Hu24 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
MAA519Hu26 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
MAA519Hu23 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
MAA519Hu25 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
MAA519Hu29 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
RAA519Hu21 载脂蛋白A1(APOA1)重组抗体 WB; IHC; ICC.
RAA519Hu27 载脂蛋白A1(APOA1)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAA519Hu22 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
FAA519Hu02 抗载脂蛋白A1(APOA1)单克隆抗体 Flow cytometry.
LAA519Hu76 载脂蛋白A1(APOA1)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA519Hu 载脂蛋白A1(APOA1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA519Hu 抗载脂蛋白A1抗体(Anti-APOA1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA519Hu 载脂蛋白A1(APOA1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA519Hu 载脂蛋白A1(APOA1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSA519Hu01 载脂蛋白A1(APOA1)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA519Hu01 载脂蛋白A1(APOA1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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