精氨酸酶(ARG)活性蛋白

Active Arginase (ARG)

ARG1; Arginase I; Liver Arginase

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 精氨酸酶(ARG)活性蛋白 产品包装(模拟)
  • 精氨酸酶(ARG)活性蛋白 产品包装(模拟)
  • APB120Mu01.jpg SDS-PAGE图
  • 精氨酸酶(ARG)活性蛋白 Figure. Western Blot; Sample: Recombinant Arg, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of Arg, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse Arg and recombinant mouse UCHL5. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用
RPB120Mu02 精氨酸酶(ARG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB120Mu01 精氨酸酶(ARG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB120Mu01 精氨酸酶(ARG)活性蛋白 Cell culture; Activity Assays.
PAB120Mu01 精氨酸酶(ARG)多克隆抗体 WB; IHC; ICC; IP.
PAB120Mu02 精氨酸酶(ARG)多克隆抗体 WB; IHC; ICC; IP.
LAB120Mu81 精氨酸酶(ARG)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB120Mu71 精氨酸酶(ARG)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB120Mu 精氨酸酶(ARG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB120Mu 精氨酸酶(ARG)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB120Mu01 精氨酸酶(ARG)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".
留言咨询