钙蛋白酶1(CAPN1)活性蛋白

Active Calpain 1 (CAPN1)

PIG30; CANPL1; MuCANP; MuCL; Calpain 1, Large Subunit; Calpain-1 Catalytic Subunit; Calcium-Dependent Protease 1; Calcium-activated neutral proteinase 1; Calpain mu-type; Micromolar-calpain

  • 钙蛋白酶1(CAPN1)活性蛋白 产品包装(模拟)
  • 钙蛋白酶1(CAPN1)活性蛋白 产品包装(模拟)
  • 钙蛋白酶1(CAPN1)活性蛋白 Gene sequencing
  • APB964Ra01.jpg SDS-PAGE图
  • 钙蛋白酶1(CAPN1)活性蛋白 Figure. Western Blot; Sample: Recombinant CAPN1, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APB964Ra01 钙蛋白酶1(CAPN1)活性蛋白 Cell culture; Activity Assays.
APB964Ra02 钙蛋白酶1(CAPN1)活性蛋白 Cell culture; Activity Assays.
RPB964Ra01 钙蛋白酶1(CAPN1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB964Ra02 钙蛋白酶1(CAPN1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB964Ra01 钙蛋白酶1(CAPN1)多克隆抗体 WB; IHC; ICC; IP.
PAB964Ra02 钙蛋白酶1(CAPN1)多克隆抗体 WB; IHC; ICC; IP.
LAB964Ra71 钙蛋白酶1(CAPN1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB964Ra21 钙蛋白酶1(CAPN1)单克隆抗体 WB; IHC; ICC; IP.
SEB964Ra 钙蛋白酶1(CAPN1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB964Ra 钙蛋白酶1(CAPN1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Bulletin du Cancer Calpain 1 in bronchoalveolar lavage fluid is associated with poor prognosis in lepidic predominant pulmonary adenocarcinoma [Pubmed: 30683309]
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