羧酸酯酶1(CES1)活性蛋白

Active Carboxylesterase 1 (CES1)

ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1

  • 羧酸酯酶1(CES1)活性蛋白 产品包装(模拟)
  • 羧酸酯酶1(CES1)活性蛋白 产品包装(模拟)
  • 羧酸酯酶1(CES1)活性蛋白 Figure. Gene Sequencing (Extract)
  • APC374Mu01.jpg Figure. SDS-PAGE
  • 羧酸酯酶1(CES1)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

carboxylesterase 1(CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant mouse CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, 150 mM NaCl, pH 7.5( Assay Buffer), ainitiated by addition 50 μL of various concentrations of CES1(dilute by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.

用法

Reconstitute in ddH2O to a concentration of 0.1-0.2 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
APC374Mu02 羧酸酯酶1(CES1)活性蛋白 Cell culture; Activity Assays.
APC374Mu61 羧酸酯酶1(CES1)活性蛋白 Cell culture; Activity Assays.
EPC374Mu61 羧酸酯酶1(CES1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC374Mu02 羧酸酯酶1(CES1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC374Mu01 羧酸酯酶1(CES1)活性蛋白 Cell culture; Activity Assays.
RPC374Mu01 羧酸酯酶1(CES1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC374Mu01 羧酸酯酶1(CES1)多克隆抗体 WB; IHC; ICC; IP.

参考文献

杂志 参考文献
International Journal of Nanomedicine Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug Interactions [Pubmed: 32764932]
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