趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白

Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)

NTN; ABCD3; C3Xkine; CXC3; CXC3C; NTT; SCYD1; ABCD3; FKN; Neurotactin; Fractalkine; Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1

  • 趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白 产品包装(模拟)
  • 趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白 产品包装(模拟)
  • 趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白 Figure. Gene Sequencing (Extract)
  • APA040Ra01.jpg Figure. SDS-PAGE
  • 趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The chemotactic effect of CX3CL1 on THP-1 cells
Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) also known as fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CX3CL1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (150uL cell suspension, 106 cells/mL in RPMI 1640 with FBS free) and SLC (15.625ng/mL, 31.25ng/mL, 62.5ng/mL and 125ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows CX3CL1 is able to induce migration of THP-1 cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of CX3CL1 occurs at 15.625-125ng/mL.
(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 31.25ng/mL CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h;
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.

Figure. The chemotactic effect of CX3CL1 on THP-1 cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APA040Ra01 趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白 Cell culture; Activity Assays.
RPA040Ra01 趋化因子C-X3-C-基元配体1(CX3CL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA040Ra01 趋化因子C-X3-C-基元配体1(CX3CL1)多克隆抗体 WB; IHC; ICC; IP.
LAA040Ra81 趋化因子C-X3-C-基元配体1(CX3CL1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA040Ra71 趋化因子C-X3-C-基元配体1(CX3CL1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA040Ra21 趋化因子C-X3-C-基元配体1(CX3CL1)单克隆抗体 WB; IHC; ICC; IP.
SEA040Ra 趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA040Ra 趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA040Ra 趋化因子C-X3-C-基元配体1(CX3CL1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
World Journal of Gastroenterology Preliminary study correlating CX3CL1/CX3CR1 expression with gastric carcinoma and gastric carcinoma perineural invasion [NCBI: PMC3989981]
International Journal of Colorectal Disease Downregulation of CX3CR1 ameliorates experimental colitis: evidence for CX3CL1-CX3CR1-mediated immune cell recruitment. [pubmed:27942903]
Frontiers in Cellular and Infection Microbiology Reduced CX3CL1 Secretion Contributes to the Susceptibility of Oral Leukoplakia-Associated Fibroblasts to Candida albicans. [pubmed:27891323]
Blood Coagul Fibrinolysis Low shear stress upregulates the expression of fractalkine through the activation of mitogen-activated protein kinases in endothelial cells [Pubmed:29406386]
Journal of Neuroinflammation The prenatal challenge with lipopolysaccharide and polyinosinic: polycytidylic acid disrupts CX3CL1-CX3CR1 and CD200-CD200R signalling in the brains of … [Pubmed: 32829711]
Cells Maternal Immune Activation Sensitizes Male Offspring Rats to Lipopolysaccharide-Induced Microglial Deficits Involving the Dysfunction of CD200–CD200R and … [Pubmed: 32664639]
Journal of Neuroinflammation CX3CR1-microglia mediates neuroinflammation and blood pressure regulation in the nucleus tractus solitarii of fructose-induced hypertensive rats [Pubmed: 32532282]
JOURNAL OF PERIODONTAL RESEARCH Potential biomarkers reflecting inflammation in patients with severe periodontitis: Fractalkine (CX3CL1) and its receptor (CX3CR1) [33641164]
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