丝切蛋白1(CFL1)活性蛋白

Active Cofilin 1 (CFL1)

CFL; p18; N-Cofilin; 18 kDa phosphoprotein; Cofilin 1, Non Muscle

  • 丝切蛋白1(CFL1)活性蛋白 产品包装(模拟)
  • 丝切蛋白1(CFL1)活性蛋白 产品包装(模拟)
  • APB559Hu01.png Figure. SDS-PAGE
  • 丝切蛋白1(CFL1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of CFL1 with ACTb.
Cofilin 1 (non-muscle; n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. Besides, Actin Beta (ACTb) has been identified as an interactor of CFL1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CFL1 and recombinant human ACTb. Briefly, CFL1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CFL1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CFL1 and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB559Hu01 丝切蛋白1(CFL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB559Hu01 丝切蛋白1(CFL1)活性蛋白 Cell culture; Activity Assays.
PAB559Hu01 丝切蛋白1(CFL1)多克隆抗体 WB; IHC; ICC; IP.
FAB559Hu51 抗丝切蛋白1(CFL1)多克隆抗体(别藻蓝蛋白标记) FCM
MAB559Hu21 丝切蛋白1(CFL1)单克隆抗体 WB; IHC; ICC; IP.
MAB559Hu22 丝切蛋白1(CFL1)单克隆抗体 WB; IHC; ICC/IF
MAB559Hu23 丝切蛋白1(CFL1)单克隆抗体 WB; IHC; ICC; IP.
MAB559Hu27 丝切蛋白1(CFL1)单克隆抗体 WB; IHC; ICC; IP.
MAB559Hu24 丝切蛋白1(CFL1)单克隆抗体 WB; IHC; ICC; IP.
MAB559Hu25 丝切蛋白1(CFL1)单克隆抗体 WB; IHC; ICC; IP.
SEB559Hu 丝切蛋白1(CFL1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB559Hu 丝切蛋白1(CFL1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB559Hu 丝切蛋白1(CFL1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Brazilian Journal of Medical and Biological Research Detection of sputum cofilin-1 as indicator of malignancy [Pubmed:29846436]
Nutrients Hydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia [Pubmed: 32784915]
The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer []
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