冷诱导RNA结合蛋白(CIRBP)活性蛋白

Active Cold Inducible RNA Binding Protein (CIRBP)

CIRP; A18HNRNP; Glycine-Rich RNA Binding Protein

  • 冷诱导RNA结合蛋白(CIRBP)活性蛋白 产品包装(模拟)
  • 冷诱导RNA结合蛋白(CIRBP)活性蛋白 产品包装(模拟)
  • 冷诱导RNA结合蛋白(CIRBP)活性蛋白 Figure . Gene Sequencing (extract)
  • APG886Mu01.png Figure. SDS-PAGE
  • 冷诱导RNA结合蛋白(CIRBP)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Cold-inducible RNA-binding protein (CIRBP) plays a critical role in controlling the cellular response upon confronting a variety of cellular stresses, including short wavelength ultraviolet light, hypoxia, and hypothermia. Besides, Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1) has been identified as an interactor of CIRBP, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse CIRBP and recombinant mouse HNRPA1. Briefly, CIRBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to HNRPA1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CIRBP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and HNRPA1 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of CIRBP with HNRPA1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APG886Mu01 冷诱导RNA结合蛋白(CIRBP)活性蛋白 Cell culture; Activity Assays.
RPG886Mu01 冷诱导RNA结合蛋白(CIRBP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
SEG886Mu 冷诱导RNA结合蛋白(CIRBP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
J Cell Mol Med Normothermic machine perfusion attenuates hepatic ischaemia‐reperfusion injury by inhibiting CIRP‐mediated oxidative stress and mitochondrial fission [34786826]
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