Ⅵ型胶原α1(COL6a1)活性蛋白

Active Collagen Type VI Alpha 1 (COL6a1)

COL6-A1; OPLL; Collagen Alpha-1(VI)chain

  • Ⅵ型胶原α1(COL6a1)活性蛋白 产品包装(模拟)
  • Ⅵ型胶原α1(COL6a1)活性蛋白 产品包装(模拟)
  • APC150Mu03.png Figure. SDS-PAGE
  • Ⅵ型胶原α1(COL6a1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of COL6a1 with RTBDN.
The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The protein encoded by COL6a1 gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. Besides, Retbindin (RTBDN) has been identified as an interactor of COL6a1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse COL6a1 and recombinant mouse RTBDN. Briefly, COL6a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RTBDN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-COL6a1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of COL6a1 and RTBDN was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APC150Mu03 Ⅵ型胶原α1(COL6a1)活性蛋白 Cell culture; Activity Assays.
RPC150Mu02 Ⅵ型胶原α1(COL6a1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC150Mu03 Ⅵ型胶原α1(COL6a1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC150Mu01 Ⅵ型胶原α1(COL6a1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC150Mu02 Ⅵ型胶原α1(COL6a1)活性蛋白 Cell culture; Activity Assays.
PAC150Mu01 Ⅵ型胶原α1(COL6a1)多克隆抗体 WB; IHC; ICC; IP.
PAC150Mu03 Ⅵ型胶原α1(COL6a1)多克隆抗体 WB; IHC; ICC; IP.
PAC150Mu02 Ⅵ型胶原α1(COL6a1)多克隆抗体 WB; IHC; ICC; IP.
LAC150Mu71 Ⅵ型胶原α1(COL6a1)多克隆抗体(生物素标记) WB; IHC; ICC.
SEC150Mu Ⅵ型胶原α1(COL6a1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC150Mu Ⅵ型胶原α1(COL6a1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Molecular & Cellular Proteomics Integrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer Biomarkers [PubMed: PMC3205865]
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