巨噬细胞集落刺激因子(MCSF)活性蛋白

Active Colony Stimulating Factor 1, Macrophage (MCSF)

CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1

  • 巨噬细胞集落刺激因子(MCSF)活性蛋白 产品包装(模拟)
  • 巨噬细胞集落刺激因子(MCSF)活性蛋白 产品包装(模拟)
  • 巨噬细胞集落刺激因子(MCSF)活性蛋白 Gene sequencing
  • APA090Mu01.jpg SDS-PAGE图
  • 巨噬细胞集落刺激因子(MCSF)活性蛋白 Figure. Western Blot; Sample: Recombinant MCSF, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1,is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Besides, Colony Stimulating Factor Receptor, Macrophage (M-CFS-R) has been identified as an interactor of M-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse M-CSF and recombinant mouse MCFSR Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to M-CFS-R-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of M-CSF and M-CFS-R was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA090Mu01 巨噬细胞集落刺激因子(MCSF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA090Mu02 巨噬细胞集落刺激因子(MCSF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA090Mu01 巨噬细胞集落刺激因子(MCSF)活性蛋白 Cell culture; Activity Assays.
PAA090Mu01 巨噬细胞集落刺激因子(MCSF)多克隆抗体 WB; IHC; ICC; IP.
PAA090Mu02 巨噬细胞集落刺激因子(MCSF)多克隆抗体 WB; IHC; ICC; IP.
SEA090Mu 巨噬细胞集落刺激因子(MCSF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA090Mu 巨噬细胞集落刺激因子(MCSF)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA090Mu01 巨噬细胞集落刺激因子(MCSF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It(ELISA Kit) Yourself"

参考文献

杂志 参考文献
Brain Pathology Microglia-Derived Macrophage Colony Stimulating Factor Promotes Generation of Proinflammatory Cytokines by Astrocytes in the Periventricular White Matter in the Hypoxic Neonatal Brain [Wiley: source]
Oncotarget miR-1207-5p suppresses lung cancer growth and metastasis by targeting CSF1 [pubmed:27107415]
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