粒细胞巨噬细胞集落刺激因子(GMCSF)活性蛋白
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
CSF2; GM-CSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor
- 编号APA045Hu61
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点5.2
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1824 ¥ 4560 ¥ 9120 ¥ 27360 ¥ 68400
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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Figure. Western Blot; Sample: APA045Hu61; Antibody: PAA045Hu06
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通过ISO 9001、ISO 13485质量体系认证
活性实验
TF-1, the human erythroleukemia cell line,provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. In house data was obtained by the following experiment: TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope everyday .
Cell proliferation of TF1 cells after incubation with GM-CSF (10ng/mL) for 3 days was shown in Figure 1.
Cell differentiation of U937 cells after incubation with GM-CSF (10ng/mL) for 5 days was shown in Figure 2.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| EPA045Hu61 | 粒细胞巨噬细胞集落刺激因子(GMCSF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA045Hu61 | 粒细胞巨噬细胞集落刺激因子(GMCSF)活性蛋白 | Cell culture; Activity Assays. |
| APA045Hu01 | 粒细胞巨噬细胞集落刺激因子(GMCSF)活性蛋白 | Cell culture; Activity Assays. |
| RPA045Hu01 | 粒细胞巨噬细胞集落刺激因子(GMCSF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA045Hu01 | 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA045Hu06 | 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA045Hu22 | 粒细胞巨噬细胞集落刺激因子(GMCSF)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA045Hu | 粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA045Hu | 粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA045Hu01 | 粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Toxicology and Environmental Health | Effects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic System [PubMed: 20391130] |
| 5 | Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trial [Pubmed:24525316] |
