半胱氨酸蛋白酶抑制剂3(Cys-C)活性蛋白

Active Cystatin C (Cys-C)

CST3; Cystatin 3; Gamma Trace; Post-Gamma-Globulin; Neuroendocrine Basic Polypeptide; Amyloid Angiopathy And Cerebral Hemorrhage

  • 半胱氨酸蛋白酶抑制剂3(Cys-C)活性蛋白 产品包装(模拟)
  • 半胱氨酸蛋白酶抑制剂3(Cys-C)活性蛋白 产品包装(模拟)
  • APA896Ra61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Cystatin C is a member of family 2 of the Cystatin superfamily. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant (Leu68 to Gln) is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage. Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate. The activity of recombinant rat Cystatin C was measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC in the assay buffer 50 mM Tris, pH 7.0. Papain was diluted to 500 ug/ml in activation buffer 50 mM Tris, 5 mM DTT, pH 7.0 and incubated at room temperature for 15 minutes. The activated papain was diluted to 100 ug/ml in the assay buffer and 20 ul different concentrations of recombinant rat Cystatin C (MW: 14.93 KD) was incubated with 20 ul 100 ug/ml papain at 37 ℃ for 10 minutes. Loading 50 µL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 µL of 200 µM substrate. Include a substrate blank containing 50 µL of assay buffer and 50 µL of 200 µM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat Cystatin C significantly decreased papain activity. The inhibition IC50 was <80 nM.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA896Ra01 半胱氨酸蛋白酶抑制剂3(Cys-C)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA896Ra61 半胱氨酸蛋白酶抑制剂3(Cys-C)活性蛋白 Cell culture; Activity Assays.
EPA896Ra61 半胱氨酸蛋白酶抑制剂3(Cys-C)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA896Ra01 半胱氨酸蛋白酶抑制剂3(Cys-C)多克隆抗体 WB; IHC; ICC; IP.
LAA896Ra71 半胱氨酸蛋白酶抑制剂3(Cys-C)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA896Ra21 半胱氨酸蛋白酶抑制剂3(Cys-C)单克隆抗体 WB; IHC; ICC; IP.
SEA896Ra 半胱氨酸蛋白酶抑制剂3(Cys-C)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA896Ra 半胱氨酸蛋白酶抑制剂3(Cys-C)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
BioMed Research International The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies [Hindawi: 740105]
Critical Care Endothelial dysfunction and renal fibrosis in endotoxemia-induced oliguric kidney injury: possible role of LPS-binding protein [Pubmed:25261195]
РОЛЬ АПОПТОЗ ИНДУЦИРУЮЩЕГО ФАКТОРА И ОКИСЛИТЕЛЬНОГО СТРЕССА ПРИ ХРОНИЧЕСКОЙ СЕРДЕЧНОЙ НЕДОСТАТОЧНОСТИ [616-005.8]
Journal of Agricultural and Food Chemistry Protective Effects of DHA-PC against Vancomycin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis in BALB/c Mice [pubmed:29254330]
THERAPEUTIC ADVANCES IN RESPIRATORY DISEASE Association of cathepsin B and cystatin C with an age-related pulmonary subclinical state in a healthy Chinese population [Pubmed: 32401159]
RSC Adv SERS based Y-shaped aptasensor for early diagnosis of acute kidney injury [Pubmed:35733690]
Food Funct A Compared Study of Gentle Ketogenic Diet Containing Medium-Chain Triglycerides or Long-Chain Triglycerides on Chronic Sleep Deprivation-Induced Cognitive … [Pubmed:35141738]
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