胰凝乳蛋白酶C(CTRC)活性蛋白

Active Chymotrypsin C (CTRC)

ELA4; CTRC; Elastase 4 ; Caldecrin

  • 胰凝乳蛋白酶C(CTRC)活性蛋白 产品包装(模拟)
  • 胰凝乳蛋白酶C(CTRC)活性蛋白 产品包装(模拟)
  • APB310Ra01.jpg SDS-PAGE图
  • 胰凝乳蛋白酶C(CTRC)活性蛋白 Figure. Western Blot; Sample: Recombinant CTRC, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Chymotrypsin C (CTRC) is a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. It regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of CTRC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CTRC and recombinant rat uPAR. Briefly, CTRC were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti- CTRC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of CTRC and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB310Ra01 胰凝乳蛋白酶C(CTRC)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB310Ra01 胰凝乳蛋白酶C(CTRC)活性蛋白 Cell culture; Activity Assays.
PAB310Ra01 胰凝乳蛋白酶C(CTRC)多克隆抗体 WB; IHC; ICC; IP.
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