内分泌腺来源血管内皮生长因子(EG-VEGF)活性蛋白

Active Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF)

PK1; EGVEGF; PRK1, PROK1; Prokineticin 1; Black Mamba Toxin-Related Protein; Mambakine

  • 内分泌腺来源血管内皮生长因子(EG-VEGF)活性蛋白 产品包装(模拟)
  • 内分泌腺来源血管内皮生长因子(EG-VEGF)活性蛋白 产品包装(模拟)
  • APA024Hu01.png Figure. SDS-PAGE
  • 内分泌腺来源血管内皮生长因子(EG-VEGF)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure 1. Cell proliferation of ECV-304 cells after stimulated with EG-VEGF.
Endothelial gland-derived VEGF (EG-VEGF) is an angiogenic protein that is structurally unrelated to VEGF. It is expressed in steroidogenic tissues such as adrenal gland, ovary, testis, and placenta. Like VEGF it can induce fenestrae in endothelial cells. To test the effect of EG-VEGF on cell proliferation of ECV-304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EG-VEGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37℃.
(A) Unstimulated ECV-304 cells cultured in 1640 for 96h;
(B) ECV-304 cells cultured in 1640, stimulated with 10ng/mL VEGF121 for 96h.

Figure. Cell proliferation of ECV-304 cells after stimulated with EG-VEGF

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA024Hu01 内分泌腺来源血管内皮生长因子(EG-VEGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA024Hu01 内分泌腺来源血管内皮生长因子(EG-VEGF)活性蛋白 Cell culture; Activity Assays.
PAA024Hu01 内分泌腺来源血管内皮生长因子(EG-VEGF)多克隆抗体 WB; IHC; ICC; IP.
MAA024Hu22 内分泌腺来源血管内皮生长因子(EG-VEGF)单克隆抗体 WB; IHC; ICC; IP.
SEA024Hu 内分泌腺来源血管内皮生长因子(EG-VEGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA024Hu 内分泌腺来源血管内皮生长因子(EG-VEGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Tumor Biology Hypoxia-induced secretion of IL-14 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
留言咨询