内皮细胞特异分子1(ESM1)活性蛋白

Active Endothelial Cell Specific Molecule 1 (ESM1)

Endocan

  • 内皮细胞特异分子1(ESM1)活性蛋白 产品包装(模拟)
  • 内皮细胞特异分子1(ESM1)活性蛋白 产品包装(模拟)
  • 内皮细胞特异分子1(ESM1)活性蛋白 Gene sequencing
  • APC463Hu01.jpg SDS-PAGE图
  • 内皮细胞特异分子1(ESM1)活性蛋白 Figure. Western Blot; Sample: Recombinant ESM1, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Endothelial cell-specific molecule 1 (ESM1) is a proteoglycan secreted by endothelial cells (primarily in the human lung and kidney tissues) and its mRNA expression is regulated by inflammatory cytokines. Endocan expression, which detected in various epithelia and adipocytes has been shown to be upregulated by vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF-2), TNF alpha, IL1 beta, or lipopolysaccharide and downregulated by IFN gamma. Genetically engineered cells overexpressing ESM1 induce tumor formation, implying that ESM1 might be involved in the pathophysiology of tumor growth in vivo. Besides, Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) has been identified as an interactor of ESM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ESM1 and recombinant human LFA1a. Briefly, ESM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LFA1a-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti- ESM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ESM1 and LFA1a was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APC463Hu01 内皮细胞特异分子1(ESM1)活性蛋白 Cell culture; Activity Assays.
RPC463Hu01 内皮细胞特异分子1(ESM1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC463Hu01 内皮细胞特异分子1(ESM1)多克隆抗体 WB; IHC; ICC; IP.
LAC463Hu71 内皮细胞特异分子1(ESM1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC463Hu22 内皮细胞特异分子1(ESM1)单克隆抗体 WB; IHC; ICC; IP.
SEC463Hu 内皮细胞特异分子1(ESM1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Urology Detection on dynamic changes of endothelial cell specific molecule-1 in acute rejection after renal transplantation [PubMed: 22608803]
Microvasc Res. Endocan is useful biomarker of survival and severity in sepsis. [Pubmed: 24769132]
J Matern Fetal Neonatal Med. Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromes [Pubmed:25211383]
Microvasc Res. Endocan is useful biomarker of survival and severity in sepsis [Pubmed:24769132]
Angiology Endocan Levels and Subclinical Atherosclerosis in Patients With Systemic Lupus Erythematosus [PubMed: 26614790]
Archives of Medical Science - Atherosclerotic Diseases Assessment of subclinical cardiac damage in chronic plaque psoriasis patients: a case control study [Journal:-102]
Int Immunopharmacol.  Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension. [pubmed:27912147]
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