红细胞生成素(EPO)活性蛋白

Active Erythropoietin (EPO)

EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

  • 红细胞生成素(EPO)活性蛋白 产品包装(模拟)
  • 红细胞生成素(EPO)活性蛋白 产品包装(模拟)
  • APA028Hu01.png Figure. SDS-PAGE
  • 红细胞生成素(EPO)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. Erythropoietin is an essential hormone for red blood cell production. EPO can cooperate with various other growth factors involved in the development of erythroid lineage from multipotent progenitors. To test the effect of EPO on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human EPO. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of TF-1 cells after incubation with EPO for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant EPO for 72h. The result was shown in Figure 2. It was obvious that EPO significantly increased cell viability of TF-1 cells.
(A) TF-1 cells cultured in 1640, stimulated with 5ng/mL EPO for 72h;
(B) Unstimulated TF-1 cells cultured in1640 for 72h.
Figure. Cell proliferation of TF-1 cells after stimulated with EPO.

Figure. Cell proliferation of TF-1 cells after stimulated with EPO

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA028Hu61 红细胞生成素(EPO)活性蛋白 Cell culture; Activity Assays.
EPA028Hu61 红细胞生成素(EPO)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA028Hu01 红细胞生成素(EPO)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA028Hu01 红细胞生成素(EPO)活性蛋白 Cell culture; Activity Assays.
APA028Hu62 红细胞生成素(EPO)活性蛋白 Cell culture; Activity Assays.
PAA028Hu06 红细胞生成素(EPO)多克隆抗体 WB; IHC; ICC; IP.
PAA028Hu01 红细胞生成素(EPO)多克隆抗体 WB; IHC; ICC; IP.
LAA028Hu71 红细胞生成素(EPO)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA028Hu23 红细胞生成素(EPO)单克隆抗体 WB; IHC; ICC; IP.
MAA028Hu26 红细胞生成素(EPO)单克隆抗体 WB; IHC; ICC; IP.
MAA028Hu21 红细胞生成素(EPO)单克隆抗体 WB; IHC; ICC; IP.
MAA028Hu24 红细胞生成素(EPO)单克隆抗体 WB; IHC; ICC; IP.
MAA028Hu25 红细胞生成素(EPO)单克隆抗体 WB; IHC; ICC; IP.
MAA028Hu22 红细胞生成素(EPO)单克隆抗体 WB,IHC
SEA028Hu 红细胞生成素(EPO)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA028Hu 红细胞生成素(EPO)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA028Hu 红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA028Hu01 红细胞生成素(EPO)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Experimental & Molecular Medicine Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression [PubMed: PMC3509184]
Biochem Biophys Res Commun. Reevaluation of erythropoietin production by the nephron [Pubmed:24832733]
Nutrition. Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. [Pubmed:2528040]
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Expert Rev Proteomics Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone [PubMed: 26496240]
Oncotarget KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin [pubmed:26575329]
international journal of molecular sciences Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. [pubmed:28216608]
Veterinary Clinical Pathology Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease [pubmed:28186645]
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease]
Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463]
PLoS Neglected Tropical Diseases Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis [pubmed:28489854]
Neurochemistry International Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia [S0197018617304783]
Pesquisa Veterinária Brasileira Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease [10.1590/s0100-736x2017000600011 ]
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Neurochemistry International Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on … [Pubmed:29180303]
Clinical and Experimental Medicine Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502]
Bulletin of Experimental Biology and Medicine Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia [Pubmed:29308565]
Bulletin of Experimental Biology and Medicine Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … [Pubmed: 30353332]
FASEB JOURNAL High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans []
Journal of Functional Foods Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats []
博士论文 PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE []
Nutrition Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind … [Pubmed: 31743809]
BRITISH JOURNAL OF HAEMATOLOGY Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans [Pubmed: 32866306]
JCI Insight. Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes [Pubmed: 32759495]
Blood Cells, Molecules, and Diseases EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness [Pubmed: 32470757]
Int J Endocrinol miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia [34367277]
J Mater Sci Mater Med Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules [Pubmed:35072831]
Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells []
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