脂肪酸结合蛋白 4(FABP4)活性蛋白

Active Fatty Acid Binding Protein 4 (FABP4)

A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein

  • 脂肪酸结合蛋白 4(FABP4)活性蛋白 产品包装(模拟)
  • 脂肪酸结合蛋白 4(FABP4)活性蛋白 产品包装(模拟)
  • 脂肪酸结合蛋白 4(FABP4)活性蛋白 Figure. Gene Sequencing (Extract)
  • APB693Hu61.png Figure. SDS-PAGE
  • 脂肪酸结合蛋白 4(FABP4)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Fatty acid binding protein-4 (FABP4) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner. FABP4 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length. Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. Lipase, Hormone Sensitive (LIPE) has been identified as an interactor of FABP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FABP4 and recombinant human LIPE. Briefly, FABP4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LIPE-coated microtiter wells and incubated for 1h at 37℃. Wells were aspirated and incubated for 1h with anti-FABP4 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of FABP4 and LIPE was shown in Figure 1, and this effect was in a dose dependent manner. The EC50 for this effect is 4.23 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
EPB693Hu61 脂肪酸结合蛋白 4(FABP4)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB693Hu62 脂肪酸结合蛋白 4(FABP4)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB693Hu01 脂肪酸结合蛋白 4(FABP4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB693Hu61 脂肪酸结合蛋白 4(FABP4)活性蛋白 Cell culture; Activity Assays.
PAB693Hu06 脂肪酸结合蛋白 4(FABP4)多克隆抗体 WB; IHC; ICC; IP.
PAB693Hu01 脂肪酸结合蛋白 4(FABP4)多克隆抗体 WB; IHC; ICC; IP.
FAB693Hu01 抗脂肪酸结合蛋白 4(FABP4)多克隆抗体 Flow cytometry.
LAB693Hu71 脂肪酸结合蛋白 4(FABP4)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB693Hu21 脂肪酸结合蛋白 4(FABP4)单克隆抗体 WB; IHC; ICC; IP.
MAB693Hu23 脂肪酸结合蛋白 4(FABP4)单克隆抗体 WB; IHC; ICC; IP.
MAB693Hu24 脂肪酸结合蛋白 4(FABP4)单克隆抗体 WB; IHC; ICC; IP.
MAB693Hu25 脂肪酸结合蛋白 4(FABP4)单克隆抗体 WB; IHC; ICC; IP.
MAB693Hu27 脂肪酸结合蛋白 4(FABP4)单克隆抗体 WB; IHC; ICC; IP.
MAB693Hu29 脂肪酸结合蛋白 4(FABP4)单克隆抗体 WB; IHC; ICC; IP.
MAB693Hu22 脂肪酸结合蛋白 4(FABP4)单克隆抗体 WB; IHC.
FAB693Hu02 抗脂肪酸结合蛋白 4(FABP4)单克隆抗体 Flow cytometry.
FAB693Hu06 抗脂肪酸结合蛋白 4(FABP4)单克隆抗体 Flow cytometry.
SEB693Hu 脂肪酸结合蛋白 4(FABP4)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB693Hu 脂肪酸结合蛋白 4(FABP4)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Clinical and Molecular Hepatology Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASH [PubMed: 23091808]
journal of Human Hypertension Siesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adults [PubMed: 26134622]
genetics and molecular research Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice [PubMed: 25867423]
Food Chem Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes [PubMed: 25952883]
Placenta Increased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsia [Pubmed:26992681]
Clin Chim Acta. Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies. [pubmed:28161274]
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … [Pubmed:29849871]
Nutrition & Diabetes Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416]
American Journal of Cancer Research 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4 [Pubmed: 31720079]
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … [Pubmed:35212074]
cells The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure [Pubmed:35455943]
Bone Research FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis [Pubmed:35729106]
留言咨询