Active Fibroblast Growth Factor 1, Acidic (FGF1)
ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 97%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 1g (保证有货 万一缺货 免费重做)
- 价格 ¥ 1200 ¥ 3000 ¥ 6000 ¥ 18000 ¥ 450000
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant FGF1, Human.
- 通过ISO 9001、ISO 13485质量体系认证
FGF1 (Fibroblast growth factor 1) is a member of FGF family, which plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts, besides, human FGF1 and mouse FGF1 shares similarities in amino acid sequence with the identity of 96%. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant human FGF1 using 3T3 fibroblasts. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. The result was shown in Figure 2. It was obvious that FGF1 significantly increased cell viability of 3T3 cells.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|RPA032Hu01||酸性成纤维细胞生长因子(FGF1)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA032Hu01||酸性成纤维细胞生长因子(FGF1)活性蛋白||Cell culture; Activity Assays.|
|PAA032Hu01||酸性成纤维细胞生长因子(FGF1)多克隆抗体||WB; IHC; ICC; IP.|
|LAA032Hu81||酸性成纤维细胞生长因子(FGF1)多克隆抗体(异硫氰酸荧光素标记)||WB; IHC; ICC; IF.|
|LAA032Hu71||酸性成纤维细胞生长因子(FGF1)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAA032Hu22||酸性成纤维细胞生长因子(FGF1)单克隆抗体||WB; IHC; ICC; IP.|
|SEA032Hu||酸性成纤维细胞生长因子(FGF1)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA032Hu||酸性成纤维细胞生长因子(FGF1)检测试剂盒(化学发光免疫分析法)||Chemiluminescent immunoassay for Antigen Detection.|
|Tissue Engineering Part A||Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat [Pubmed:24814095]|