酸性成纤维细胞生长因子(FGF1)活性蛋白

Active Fibroblast Growth Factor 1, Acidic (FGF1)

ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 酸性成纤维细胞生长因子(FGF1)活性蛋白 产品包装(模拟)
  • 酸性成纤维细胞生长因子(FGF1)活性蛋白 产品包装(模拟)
  • 酸性成纤维细胞生长因子(FGF1)活性蛋白 Gene sequencing
  • APA032Hu01.jpg SDS-PAGE图
  • 酸性成纤维细胞生长因子(FGF1)活性蛋白 Figure. Western Blot; Sample: Recombinant FGF1, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

FGF1 (Fibroblast growth factor 1) is a member of FGF family, which plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts, besides, human FGF1 and mouse FGF1 shares similarities in amino acid sequence with the identity of 96%. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant human FGF1 using 3T3 fibroblasts. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. The result was shown in Figure 2. It was obvious that FGF1 significantly increased cell viability of 3T3 cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用
RPA032Hu01 酸性成纤维细胞生长因子(FGF1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA032Hu01 酸性成纤维细胞生长因子(FGF1)活性蛋白 Cell culture; Activity Assays.
PAA032Hu01 酸性成纤维细胞生长因子(FGF1)多克隆抗体 WB; IHC; ICC; IP.
LAA032Hu81 酸性成纤维细胞生长因子(FGF1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA032Hu71 酸性成纤维细胞生长因子(FGF1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA032Hu22 酸性成纤维细胞生长因子(FGF1)单克隆抗体 WB; IHC; ICC; IP.
SEA032Hu 酸性成纤维细胞生长因子(FGF1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA032Hu 酸性成纤维细胞生长因子(FGF1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Tissue Engineering Part A Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat [Pubmed:24814095]
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