成纤维细胞生长因子18(FGF18)活性蛋白

Active Fibroblast Growth Factor 18 (FGF18)

ZFGF5

  • 成纤维细胞生长因子18(FGF18)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子18(FGF18)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子18(FGF18)活性蛋白 Figure . Gene Sequencing (extract)
  • APC907Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Fibroblast Growth Factor-18 (FGF18) is a trophic factor for mature chondrocytes
and their progenitors. It has been reported to have significant anabolic effects on
cartilage. FGF18 plays a central role in skeletal growth and
development.Besides,Fibroblast Growth Factor Receptor 3 (FGFR3) has been
identified as an interactor of FGF18, thus a binding ELISA assay was conducted
to detect the interaction of recombinant human FGF18 and recombinant human
FGFR3. Briefly, FGF18 were diluted serially in PBS, with 0.01% BSA (pH 7.4).
Duplicate samples of 100μl were then transferred to FGFR3-coated microtiter
wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated
for 1h with anti-FGF18 pAb, then aspirated and washed 3 times. After incubation
with HRP labelled secondary antibody, wells were aspirated and washed 3 times.
With the addition of substrate solution, wells were incubated 15-25 minutes at
37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately.
The binding activity of FGF18 and FGFR3 was shown in Figure 1, and this effect
was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
EPC907Hu62 成纤维细胞生长因子18(FGF18)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC907Hu02 成纤维细胞生长因子18(FGF18)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC907Hu02 成纤维细胞生长因子18(FGF18)活性蛋白 Cell culture; Activity Assays.
RPC907Hu01 成纤维细胞生长因子18(FGF18)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC907Hu01 成纤维细胞生长因子18(FGF18)活性蛋白 Cell culture; Activity Assays.
PAC907Hu01 成纤维细胞生长因子18(FGF18)多克隆抗体 WB; IHC; ICC; IP.
MAC907Hu22 成纤维细胞生长因子18(FGF18)单克隆抗体 WB; IHC; ICC; IP.
SEC907Hu 成纤维细胞生长因子18(FGF18)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEC907Hu 成纤维细胞生长因子18(FGF18)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC907Hu 成纤维细胞生长因子18(FGF18)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
CCC907Hu 成纤维细胞生长因子18(FGF18)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMC907Hu 成纤维细胞生长因子18(FGF18)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Pesqui. Bras. Odontopediatria Clín. Integr Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine []
J Exp Clin Cancer Res HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway [Pubmed:35277183]
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