成纤维细胞生长因子19(FGF19)活性蛋白

Active Fibroblast Growth Factor 19 (FGF19)

  • 成纤维细胞生长因子19(FGF19)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子19(FGF19)活性蛋白 产品包装(模拟)
  • APC917Hu01.png Figure. SDS-PAGE
  • 成纤维细胞生长因子19(FGF19)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Fibroblast Growth Factor 19 (FGF19) is a member of the fibroblast growth factor (FGF) family. FGFs are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. In general, FGFs are expressed during embryonic development and in restricted adult tissues.Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites. FGF19-FGFR1 is one of paired transcripts mediating the dialogues between extraembryonic membrane and endometrium. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGF19 and recombinant human FGFR1. Briefly, FGF19 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF19 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGF19 and recombinant human FGFR1 was shown in Figure 1, the EC50 for this effect is 3.83 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
EPC917Hu61 成纤维细胞生长因子19(FGF19)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC917Hu01 成纤维细胞生长因子19(FGF19)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC917Hu61 成纤维细胞生长因子19(FGF19)活性蛋白 Cell culture; Activity Assays.
RPC917Hu02 成纤维细胞生长因子19(FGF19)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC917Hu01 成纤维细胞生长因子19(FGF19)活性蛋白 Cell culture; Activity Assays.
PAC917Hu01 成纤维细胞生长因子19(FGF19)多克隆抗体 WB; IHC
MAC917Hu21 成纤维细胞生长因子19(FGF19)单克隆抗体 WB; IHC; ICC; IP.
SEC917Hu 成纤维细胞生长因子19(FGF19)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC917Hu 成纤维细胞生长因子19(FGF19)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMC917Hu 成纤维细胞生长因子19(FGF19)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
The Journal of Nutritional Biochemistry Raspberry pomace alters cecal microbial activity and reduces secondary bile acids in rats fed a high-fat diet. [pubmed:28437712]
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