成纤维细胞生长因子22(FGF22)活性蛋白

Active Fibroblast Growth Factor 22 (FGF22)

  • 成纤维细胞生长因子22(FGF22)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子22(FGF22)活性蛋白 产品包装(模拟)
  • APC909Mu01.jpg SDS-PAGE图
  • 成纤维细胞生长因子22(FGF22)活性蛋白 Figure. Western Blot; Sample: Recombinant FGF22, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

FGF22 (Fibroblast growth factor 22) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF22 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF22. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of 3T3 cells after incubation with FGF22 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF22 for 48h. The result was shown in Figure 2. It was obvious that FGF22 significantly increased cell viability of 3T3 cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPC909Mu02 成纤维细胞生长因子22(FGF22)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC909Mu01 成纤维细胞生长因子22(FGF22)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC909Mu01 成纤维细胞生长因子22(FGF22)活性蛋白 Cell culture; Activity Assays.
PAC909Mu01 成纤维细胞生长因子22(FGF22)多克隆抗体 WB; IHC; ICC; IP.
MEC909Mu 成纤维细胞生长因子22(FGF22)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
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