成纤维细胞生长因子23(FGF23)活性蛋白

Active Fibroblast Growth Factor 23 (FGF23)

ADHR; HYPF; HPDR2; PHPTC; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor

  • 成纤维细胞生长因子23(FGF23)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子23(FGF23)活性蛋白 产品包装(模拟)
  • APA746Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Fibroblast growth factor 23 or FGF23 is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate and vitamin D metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 decreases the reabsorption and increases excretion of phosphate and suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption. Besides, Fibroblast Growth Factor Receptor 1(FGFR1) has been identified as an interactor of FGF23, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF23 and recombinant human FGFR1. Briefly, FGF23 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF23 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FGF23 and FGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in ddH2O to a concentration of 0.1-0.25 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA746Hu01 成纤维细胞生长因子23(FGF23)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA746Hu02 成纤维细胞生长因子23(FGF23)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA746Hu03 成纤维细胞生长因子23(FGF23)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA746Hu03 成纤维细胞生长因子23(FGF23)活性蛋白 Cell culture; Activity Assays.
RPA746Hu04 成纤维细胞生长因子23(FGF23)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA746Hu01 成纤维细胞生长因子23(FGF23)活性蛋白 Cell culture; Activity Assays.
PAA746Hu02 成纤维细胞生长因子23(FGF23)多克隆抗体 WB,IHC
PAA746Hu03 成纤维细胞生长因子23(FGF23)多克隆抗体 WB; IHC; ICC; IP.
PAA746Hu04 成纤维细胞生长因子23(FGF23)多克隆抗体 WB; IHC; ICC; IP.
PAA746Hu01 成纤维细胞生长因子23(FGF23)多克隆抗体 WB; IHC; ICC; IP.
LAA746Hu71 成纤维细胞生长因子23(FGF23)多克隆抗体(生物素标记) WB; IHC; ICC.
RAA746Hu21 成纤维细胞生长因子23(FGF23)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAA746Hu22 成纤维细胞生长因子23(FGF23)单克隆抗体 WB; IHC; ICC; IP.
LAA746Hu72 成纤维细胞生长因子23(FGF23)单克隆抗体(生物素标记) WB; IHC; ICC.
MEA746Hu 成纤维细胞生长因子23(FGF23)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA746Hu 成纤维细胞生长因子23(FGF23)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA746Hu 成纤维细胞生长因子23(FGF23)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Journal of international medicine Prognostic impact of renal function in precapillary pulmonary hypertension [Pubmed: 24011362]
BMC Nephrology FGF-23 associated with the progression of coronary artery calcification in hemodialysis patients [Pubmed: 24180481]
Journal of Clinical Densitometry Lower Fibroblast Growth Factor 23 Levels in Young Adults With Crohn Disease as a Possible Secondary Compensatory Effect on the Disturbance of Bone and Mineral Metabolism [Pubmed: 23623649]
J Intern Med. Prognostic impact of renal function in precapillary pulmonary hypertension. [Pubmed:24011362]
Journal of Bone and Mineral Research Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth [Pubmed:25639729]
Nefrologia Fibroblast growth factor is associated to left ventricular mass index, anemia and low values of transferrin saturation El factor de crecimiento fibroblástico está asociado con el índice de masa ventricular izquierda, anemia y niveles bajos de saturación de la transferrina [PubMed: 26394828]
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
Ann Biol Clin (Paris) Les biomarqueurs des calcifications vasculaires: quelles limites analytiques pour leur transfert de la recherche bioclinique à la pratique? [PubMed: 26069069]
Scandinavian Journal of Gastroenterology High-dose fast infusion of parenteral iron isomaltoside is efficacious in inflammatory bowel disease patients with iron-deficiency anaemia without profound changes in phosphate or fibroblast growth factor 23 [Pubmed:27326766]
PLOS ONE Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting. [pubmed:27442254]
Journal of Laboratory Diagnostics Evaluation of concentration fibroblast growth factor FGF-23 in hemodialysed patients and after kidney transplantation [publication:312592751]
Journal of Integrative Nephrology & Andrology Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients [issn:2394-2916]
Journal of Renal Nutrition ADAM17, a New Player in the Pathogenesis of Chronic Kidney Disease-Mineral and Bone Disorder. [pubmed:29056164]
Experimental Biology and Medicine(Maywood) Effect of cross-linked chitosan iron (III) on vascular calcification in uremic rats [Pubmed:29763365]
Psychiatry Research Cerebrospinal fluid FGF23 levels correlate with a measure of impulsivity [Pubmed:29677623]
BMC Pediatrics Serum and urine FGF23 and IGFBP-7 for the prediction of acute kidney injury in critically ill children [Pubmed:29907141]
Medical Science Monitor Effect of Salt Intervention on Serum Levels of Fibroblast Growth Factor 23 (FGF23) in Chinese Adults: An Intervention Study [Pubmed:29608553]
International Journal of Chronic Obstructive Pulmonary Disease Disconnection of pulmonary and systemic arterial stiffness in COPD [Pubmed:29881265]
Saudi journal of kidney diseases and transplantation Prevalence of cardiac arrhythmia and risk factors in chronic kidney disease patients [Pubmed:29970732]
Nephrology Dialysis Transplantation High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease [Pubmed: 30189026]
BioFactors  Alteration in serum concentrations of FGF19, FGF21, and FGF23 in patients with urothelial carcinoma [Pubmed: 30334297]
Biological Trace Element Research Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients [Pubmed: 30847765]
Clin Implant Dent Relat Res. Evaluation of FGF‐23 and 25(OH)D3 levels in peri‐implant sulcus fluid in peri‐implant health and diseases [Pubmed: 31407857]
JACC-Cardiovascular Imaging PREVALANCE AND PROGRESSION OF CARDIOVASCULAR CALCIFICATIONS IN HAEMODIALYSIS PATIENTS [Pubmed: 28797410]
Saudi J Kidney Dis Transpl Serum fibroblast growth factor 23 levels do not correlate with carotid intima-media thickness in patients with chronic kidney disease [Pubmed: 31696838]
Biomedicine & Pharmacotherapy MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7 [Pubmed: 31918268]
Is Serum FGF-23 Associated with Subclinic Atherosclerosis in Patients with AA Amyloidosis? []
THE RELATIONSHIP BETWEEN ARTERIAL STIFNESS AND 25-(OH) VITAMIN D, FGF 23 IN MAINTENANCE HEMODIALYSIS PATIENTS []
THE RELATIONSHIP BETWEEN ARTERIAL STIFFNESS AND 25-(OH) VITAMIN D, FGF 23 IN MAINTENANCE HEMODIALYSIS PATIENTS KRON?K ¡­ []
Calcif Tissue Int The Measurement and Interpretation of Fibroblast Growth Factor 23 (FGF23) Concentrations [Pubmed:35665817]
European Journal of Medical Genetics Genetic and clinical profile of patients with hypophosphatemic rickets [Pubmed:35738466]
African Health Sciences Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease []
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