Active Fibroblast Growth Factor 9 (FGF9)
GAF; HBFG9; Glia-Activating Factor; Heparin-Binding Growth Factor 9
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1642 ¥ 4104 ¥ 8208 ¥ 24624 ¥ 61560
- Figure. SDS-PAGE
- Figure. Western Blot
- 通过ISO 9001、ISO 13485质量体系认证
Figure. The binding activity of FGF9 with FGFR1.
Fibroblast Growth Factor 9 (FGF9) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF6, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF9 and recombinant human FGFR1. Briefly, FGF9 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of
FGF9 and FGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|RPA036Hu01||成纤维细胞生长因子9(FGF9)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA036Hu01||成纤维细胞生长因子9(FGF9)活性蛋白||Cell culture; Activity Assays.|
|PAA036Hu01||成纤维细胞生长因子9(FGF9)多克隆抗体||WB; IHC; ICC; IP.|
|LAA036Hu71||成纤维细胞生长因子9(FGF9)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAA036Hu22||成纤维细胞生长因子9(FGF9)单克隆抗体||WB; IHC; ICC; IP.|
|SEA036Hu||成纤维细胞生长因子9(FGF9)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA036Hu||成纤维细胞生长因子9(FGF9)检测试剂盒(化学发光免疫分析法)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA036Hu01||成纤维细胞生长因子9(FGF9)检测试剂盒DIY材料(酶联免疫吸附试验法)||Main materials for "Do It(ELISA Kit) Yourself"|