半乳糖凝集素4(GAL4)活性蛋白

Active Galectin 4 (GAL4)

LGALS4; Lactose-Binding Lectin 4; L-36 Lactose-Binding Protein; L36LBP; Lectin,Galactoside-Binding Soluble 4

  • 半乳糖凝集素4(GAL4)活性蛋白 产品包装(模拟)
  • 半乳糖凝集素4(GAL4)活性蛋白 产品包装(模拟)
  • APA304Bo01.png Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Galectin-4(GAL4) is a protein that in humans is encoded by the LGALS4 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant cattle GAL4 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL4 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL4, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL4 is 3.1 μg/mL.

Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
APA304Bo01 半乳糖凝集素4(GAL4)活性蛋白 Cell culture; Activity Assays.
RPA304Bo01 半乳糖凝集素4(GAL4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA304Bo01 半乳糖凝集素4(GAL4)多克隆抗体 WB; IHC; ICC; IP.
CGA304Bo01 半乳糖凝集素4(GAL4)cDNA PCR、蛋白表达等
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