谷胱甘肽S转移酶π1(GSTp)活性蛋白

Active Glutathione S Transferase Pi (GSTp)

GSTP1; GST-P1; DFN7; FAEES3; GST3

  • 谷胱甘肽S转移酶π1(GSTp)活性蛋白 产品包装(模拟)
  • 谷胱甘肽S转移酶π1(GSTp)活性蛋白 产品包装(模拟)
  • 谷胱甘肽S转移酶π1(GSTp)活性蛋白 Figure . Gene Sequencing (extract)
  • APB090Hu01.jpg Figure. SDS-PAGE
  • 谷胱甘肽S转移酶π1(GSTp)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GSTp and recombinant human CDK5. Briefly, GSTp were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100ul were then transferred to CDK5-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of of GSTp and CDK5 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPB090Hu02 谷胱甘肽S转移酶π1(GSTp)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB090Hu01 谷胱甘肽S转移酶π1(GSTp)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB090Hu01 谷胱甘肽S转移酶π1(GSTp)活性蛋白 Cell culture; Activity Assays.
APB090Hu02 谷胱甘肽S转移酶π1(GSTp)活性蛋白 Cell culture; Activity Assays.
PAB090Hu02 谷胱甘肽S转移酶π1(GSTp)多克隆抗体 WB; IHC; ICC; IP.
PAB090Hu01 谷胱甘肽S转移酶π1(GSTp)多克隆抗体 WB; IHC; ICC/IF
LAB090Hu81 谷胱甘肽S转移酶π1(GSTp)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB090Hu71 谷胱甘肽S转移酶π1(GSTp)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB090Hu21 谷胱甘肽S转移酶π1(GSTp)单克隆抗体 WB; IHC; ICC; IP.
MAB090Hu22 谷胱甘肽S转移酶π1(GSTp)单克隆抗体 WB; IHC; ICC; IP.
LAB090Hu72 谷胱甘肽S转移酶π1(GSTp)单克隆抗体(生物素标记) WB; IHC; ICC.
LAB090Hu82 谷胱甘肽S转移酶π1(GSTp)单克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
WEB090Hu 谷胱甘肽S转移酶π1(GSTp)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB090Hu 谷胱甘肽S转移酶π1(GSTp)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB090Hu 谷胱甘肽S转移酶π1(GSTp)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB090Hu01 谷胱甘肽S转移酶π1(GSTp)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料
PGB090Hu01 谷胱甘肽S转移酶π1(GSTp)引物对 PCR

参考文献

杂志 参考文献
International Journal of Medical Sciences Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117]
Mol Neurobiol Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019]
Journal of Biomaterials and Tissue Engineering 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007]
Neuroscience Bulletin Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state [Pubmed:30019218]
Arabian Journal of Chemistry Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats []
International Journal of Nature and Life Sciences The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands []
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