Gremlin 1蛋白(GREM1)活性蛋白

Active Gremlin 1 (GREM1)

PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1

  • Gremlin 1蛋白(GREM1)活性蛋白 产品包装(模拟)
  • Gremlin 1蛋白(GREM1)活性蛋白 产品包装(模拟)
  • APC128Hu01.png Figure. SDS-PAGE
  • Gremlin 1蛋白(GREM1)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Gremlin 1 (GREM1) is an inhibitor in the TGF-beta signaling pathway. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GREM1 and recombinant human BMP2. Briefly, GREM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to BMP2-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST and incubated for 1h with anti-GREM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human GREM1 and recombinant human BMP2 was shown in Figure 1，the EC50 for this effect is 0.1~0.562 ug/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APC128Hu02 Gremlin 1蛋白(GREM1)活性蛋白 Cell culture; Activity Assays.
RPC128Hu01 Gremlin 1蛋白(GREM1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC128Hu01 Gremlin 1蛋白(GREM1)活性蛋白 Cell culture; Activity Assays.
RPC128Hu02 Gremlin 1蛋白(GREM1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC128Hu01 Gremlin 1蛋白(GREM1)多克隆抗体 WB; IHC; ICC; IP.
PAC128Hu02 Gremlin 1蛋白(GREM1)多克隆抗体 WB; IHC; ICC; IP.
LAC128Hu81 Gremlin 1蛋白(GREM1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAC128Hu71 Gremlin 1蛋白(GREM1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC128Hu21 Gremlin 1蛋白(GREM1)单克隆抗体 WB; IHC; ICC; IP.
MAC128Hu23 Gremlin 1蛋白(GREM1)单克隆抗体 WB; IHC; ICC; IP.
RAC128Hu21 Gremlin 1蛋白(GREM1)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAC128Hu22 Gremlin 1蛋白(GREM1)单克隆抗体 WB,IHC
SEC128Hu Gremlin 1蛋白(GREM1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC128Hu Gremlin 1蛋白(GREM1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Lung Intrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial Hypertension [PubMed: 25926293]
Tumour Biol Overexpression of Gremlin promotes non-small cell lung cancer progression [PubMed: 26392110]
Int J Cardiol Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patients [PubMed: 26539964]
International Journal of Cardiology Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-termprognosis in suspected and proven acute heart failure patients [Pubmed:26539964]
American Journal of Respiratory Cell and Molecular Biology BMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAH [Pubmed:27187737]
J Rheumatol GREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and Invasiveness [Pubmed:26834210]
European cells & materials The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. [pubmed:28266688]
Sci Rep. Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients. [pubmed:28327551]
American Journal of Respiratory Cell and Molecular Biology Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial … [rcmb.2015-0402OC]
Molecular Medicine Reports Gremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cells [pubmed:28260028]
Cellular Haemostasis and Platelets Platelets as a novel source of Gremlin-1: Implications for thrombo inflammation [pubmed:27929199]
Scientific Reports Gremlin, A Potential Urinary Biomarker of Anca-Associated Crescentic Glomerulonephritis [Pubmed: 31053735]
International Journal of Molecular Sciences Transcriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage Regeneration [Pubmed: 31878307]
American Journal of Physiology-Cell Physiology Suppression of microRNA-23a/b-3p delays the development degenerative joint disease by activating Grem1 and TGF-β/Smad signaling pathway [Pubmed: 32186934]
Inflammopharmacology Protective role of microRNA-23a/b-3p inhibition against osteoarthritis through Gremlin1-depenent activation of TGF-β/smad signaling in chondrocytesa [Pubmed:35441352]
Mol Carcinog CAF‐derived exosomes transmitted Gremlin‐1 promotes cancer progression and decreases the sensitivity of hepatoma cells to sorafenib [Pubmed:35638711]
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