生长调节致癌基因β(GROb)活性蛋白

Active Growth Regulated Oncogene Beta (GROb)

CXCL2; SCYB2; MIP2; GRO2; MIP2a; MGSAb; CINC2a; HSF; Macrophage inflammatory protein 2-alpha; Hematopoietic synergistic factor; Chemokine(C-X-C-Motif)ligand 2

  • 生长调节致癌基因β(GROb)活性蛋白 产品包装(模拟)
  • 生长调节致癌基因β(GROb)活性蛋白 产品包装(模拟)
  • APB603Ra01.jpg SDS-PAGE图
  • 生长调节致癌基因β(GROb)活性蛋白 Figure. Western Blot; Sample: Recombinant GROb, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APB603Ra01 生长调节致癌基因β(GROb)活性蛋白 Cell culture; Activity Assays.
RPB603Ra01 生长调节致癌基因β(GROb)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB603Ra01 生长调节致癌基因β(GROb)多克隆抗体 WB; IHC; ICC; IP.
MAB603Ra21 生长调节致癌基因β(GROb)单克隆抗体 WB; IHC; ICC; IP.
SEB603Ra 生长调节致癌基因β(GROb)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
FEBS Journal Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced chemokine release in both TRAIL-resistant and TRAIL-sensitive cells via nuclear factor kappa B [PubMed: 19120450]
PLoS One. HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis [Plos: Source]
Physiol Genomics. Identification of human exercise-induced myokines using secretome analysis [Pubmed:24520153]
Diabetes Protein Inhibitor of Activated STAT 1 (PIAS1) Protects Against Obesity-Induced Insulin Resistance by Inhibiting Inflammation Cascade in Adipose Tissue [PubMed: 26324179]
Respiration Bubble CPAP support after discontinuation of mechanical ventilation protects rat lungs with ventilator-induced lung injury [Pubmed:26800273]
Scientific Reports The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37Ra [Pubmed:26912204]
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