Active Hexosaminidase A Alpha (HEXa)
TSD; Hex-A; Tay Sachs Disease; GM2 Gangliosidosis; Beta-Hexosaminidase Subunit Alpha; Beta-N-Acetylhexosaminidase Subunit Alpha; N-Acetyl-Beta-Glucosaminidase Alpha
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 97%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2004 ¥ 5010 ¥ 10020 ¥ 30060 ¥ 75150
- Figure. Western Blot; Sample: Recombinant HEXa, Mouse.
- 通过ISO 9001、ISO 13485质量体系认证
Hexosaminidase A Alpha (HEXa) is a lysosomal enzyme. There are three predominant isoenzymes: hexosaminidase A, B and S. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines. The enzymes are composed of two alpha and/or beta subunits, which are coded by HEXA and HEXB genes, respectively. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit which contains a key residue, Arg-424 is able to hydrolyze GM2 gangliosides. Hexosaminidase A (alpha polypeptide) plays a critical role in the brain and spinal cord (central nervous system). Besides, Hexosaminidase B Beta (HEXB) has been identified as an interactor of HEXA, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse HEXA and recombinant mouse HEXB. Briefly, HEXA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HEXB-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HEXA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of HEXA and HEXB was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Mus musculus (Mouse，小鼠)||应用(仅供研究使用，不用于临床诊断！)|
|APA195Mu01||氨基己糖苷酶Aα(HEXa)活性蛋白||Cell culture; Activity Assays.|
|RPA195Mu01||氨基己糖苷酶Aα(HEXa)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA195Mu02||氨基己糖苷酶Aα(HEXa)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA195Mu01||氨基己糖苷酶Aα(HEXa)多克隆抗体||WB; IHC; ICC; IP.|
|PAA195Mu02||氨基己糖苷酶Aα(HEXa)多克隆抗体||WB; IHC; ICC; IP.|
|SEA195Mu||氨基己糖苷酶Aα(HEXa)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|