组氨酸丰富糖蛋白(HRG)活性蛋白

Active Histidine Rich Glycoprotein (HRG)

HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG

  • 组氨酸丰富糖蛋白(HRG)活性蛋白 产品包装(模拟)
  • 组氨酸丰富糖蛋白(HRG)活性蛋白 产品包装(模拟)
  • 组氨酸丰富糖蛋白(HRG)活性蛋白 Gene sequencing
  • APC534Hu61.jpg SDS-PAGE图
  • 组氨酸丰富糖蛋白(HRG)活性蛋白 Figure. Western Blot;
    Sample: Recombinant HRG, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

HRG (Histidine-rich glycoprotein) is a plasma glycoprotein which is distinguished by its high content of histidine and proline. HRG binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent
metal ions. Thus, a functional binding ELISA assay was constructed to detect the association of rhHRG with heparin. Briefly, Microtiter wells were coated with OVA-conjugated-heparin. rhHRG were diluted serially in 0.01M PBS (pH 7.4). Duplicate samples of 100uL were then transferred to the coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HRG PAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate Solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately.
The binding activity of rhHRG with heparin was shown in Figure 1 and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPC534Hu01 组氨酸丰富糖蛋白(HRG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC534Hu61 组氨酸丰富糖蛋白(HRG)活性蛋白 Cell culture; Activity Assays.
EPC534Hu61 组氨酸丰富糖蛋白(HRG)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC534Hu01 组氨酸丰富糖蛋白(HRG)多克隆抗体 WB; IHC; ICC; IP.
LAC534Hu71 组氨酸丰富糖蛋白(HRG)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC534Hu24 组氨酸丰富糖蛋白(HRG)单克隆抗体 WB; IHC; ICC; IP.
MAC534Hu22 组氨酸丰富糖蛋白(HRG)单克隆抗体 WB; IHC; ICC; IP.
MAC534Hu21 组氨酸丰富糖蛋白(HRG)单克隆抗体 WB; IHC; ICC; IP.
MAC534Hu23 组氨酸丰富糖蛋白(HRG)单克隆抗体 WB; IHC; ICC; IP.
MAC534Hu26 组氨酸丰富糖蛋白(HRG)单克隆抗体 WB; IHC; ICC; IP.
SEC534Hu 组氨酸丰富糖蛋白(HRG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC534Hu 组氨酸丰富糖蛋白(HRG)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
J. Proteome Res. Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS [ACS: pr300330z]
Medical Oncology Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancer [Springer: Source]
Int J Cancer The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine‐rich glycoprotein [PubMed: 25363753]
Nucleic Acid Therapeutics Pharmacokinetic and Pharmacodynamic Investigations of ION-353382, a Model Antisense Oligonucleotide: Using Alpha-2-Macroglobulin and Murinoglobulin Double-Knockout Mice [Pubmed:27031383]
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer [y2016:0077095.html]
Hypertension Research Combination of serum histidine-rich glycoprotein and uterine artery Doppler to predict preeclampsia [Pubmed:29416108]
journal of histochemistry & cytochemistry Ultrastructural Localization of Histidine-rich Glycoprotein in Skeletal Muscle Fibers: Colocalization With AMP Deaminase [Pubmed: 31880188]
DOKLADY BIOCHEMISTRY AND BIOPHYSICS Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … [Pubmed: 32342301]
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