整合素关联蛋白(IAP)活性蛋白

Active Integrin Associated Protein (IAP)

CD47; MER6; OA3; Antigen Identified By Monoclonal Antibody 1D8; Antigenic Surface Determinant Protein OA3; Rh-Related Antigen

  • 整合素关联蛋白(IAP)活性蛋白 产品包装(模拟)
  • 整合素关联蛋白(IAP)活性蛋白 产品包装(模拟)
  • APB014Mu01.jpg SDS-PAGE图
  • 整合素关联蛋白(IAP)活性蛋白 Figure. Western Blot; Sample: Recombinant IAP, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Integrin Associated Protein (IAP) also known as Cluster of Differentiation 47 (CD47) is a transmembrane protein. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells. Besides, Signal Regulatory Protein Alpha (SIRPa) has been identified as an interactor of IAP, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IAP and recombinant mouse SIRPa. Briefly, IAP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIRPa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IAP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IAP and SIRPa was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

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编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APB014Mu01 整合素关联蛋白(IAP)活性蛋白 Cell culture; Activity Assays.
RPB014Mu01 整合素关联蛋白(IAP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB014Mu01 整合素关联蛋白(IAP)多克隆抗体 WB; IHC; ICC; IP.
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